Invitrogen™

Platinum™ II Hot-Start PCR Master Mix (2X)

Catalog number:  14000012

 Related applications:

Genotyping & Genomic Profiling

| PCR

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Description

Invitrogen Platinum II Hot-Start PCR Master Mix (2X) offers Platinum II Taq Hot-Start DNA Polymerase premixed with Platinum II PCR buffer and dNTPs for convenient PCR setup. Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and leading hot-start technology.

Features of Platinum II Hot-Start PCR Master Mix (2X) include:
Innovative buffer—enables universal annealing temperature by isostabilizing primer-template duplex structures
Engineered Taq DNA polymerase—confers fast cycling and resistance to common inhibitors
Platinum hot-start technology—enables superior specificity, sensitivity, and yields; allows for room temperature reaction setup

Platinum II Taq Hot-Start DNA Polymerase is an engineered Taq DNA polymerase that shows increased resistance to reaction inhibitors originating from sample material or DNA purification steps. The polymerase has a higher DNA synthesis rate and delivers PCR results more than two times faster than other Taq DNA polymerases. Proprietary Platinum Taq antibodies block polymerase activity at ambient temperatures and dissociate after the initial denaturation step at 94°C. This automatic "hot start" provides increased sensitivity, specificity, and yield, while allowing reaction assembly at room temperature.

Due to the unique composition of the Platinum II PCR buffer, the annealing temperature is 60°C for most primer pairs designed following the general design rules. Isostabilizing molecules in the buffer increase primer–template duplex stability during the annealing step and contribute to enhanced specificity without the need to optimize annealing temperature for each primer pair. With Platinum II Hot-Start PCR Master Mix (2X), different PCR assays can be cycled together using the same protocol with universal primer annealing temperature and the extension step selected for the longest fragment to be amplified.

Platinum II Hot-Start PCR Master Mix (2X) is provided with the optional Platinum GC Enhancer for specific amplification and improved yields of GC-rich targets.

Use Platinum II Hot-Start PCR Master Mix (2X) for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR, in applications like genotyping, high-throughput PCR, or with samples of suboptimal purity.

For further streamlining of PCR workflow, we offer Platinum II Hot-Start Green PCR Master Mix (2X), which additionally contains a density reagent and two tracking dyes for direct gel loading, for even fewer pipetting steps during PCR product analysis.

Find out more at www.thermofisher.com/platinumiitaq ›

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Polymerase: Platinum™ II Taq Hot-Start DNA Polymerase
Fidelity (vs. Taq): 1 X
GC-Rich PCR Performance: High
Reaction Format: SuperMix or Master Mix
Product Overhang: 3'-A
Reaction Speed: Fast or Standard
Final Product Size(s): 5 kb or less
Product Line: Platinum™
Product Size: 50 reactions
Shipping Condition: Dry Ice

Contents & storage

• Platinum II PCR Master Mix (2X), 1.25 mL
• Platinum GC Enhancer, 1.25 mL
• Water, nuclease-free, 1.25 mL

Store at -20°C in a non-frost-free freezer.

Documents

Manuals & protocols