Dynabeads™ M-450 Tosylactivated are 4.5 μm superparamagnetic beads containing surface tosyl groups. The beads covalently bind primary amino and sulfhydryl groups in antibodies to position them in the optimal orientation for cell-surface protein binding. Low background and covalent binding of the antibodies make these beads an excellent choice for cell separation applications when a downstream release from the beads is not required.
• Quickly deplete cells or positively isolate target cells for downstream molecular analysis • Excellent for cell isolation procedures using your own unique antibody • Several antibodies can be coupled on the same beads for non-cell-based applications, such as cell activation
Coupling procedure Covalent coupling is performed overnight by incubating the antibody of choice with the Dynabeads™ M-450 Tosylactivated beads. Optimal coupling occurs at high pH (8.5–9.5) and at 37°C. For pH-labile antibodies, coupling can be performed in an alternative buffer at pH 7.4.
Not recommended for immunoprecipitation (IP) These 4.5 μm Tosylactivated beads are not recommended for IP as they have a lower surface capacity per unit mass when compared to 1 μm and 2.8 μm Tosylactivated and Epoxy Dynabeads™.