HBSS (10X), calcium, magnesium, phenol red This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  14060040

Related applications:

Mammalian Cell Culture

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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media or for reagent preparation. We offer a variety of Gibco® HBSS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.

This HBSS is modified as follows:

• Calcium• Sodium Bicarbonate
• Magnesium
• Glucose
• Phenol Red

The complete formulation is available.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® HBSS is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Concentrated forms of Gibco® HBSS require pH adjustment and filtration at the time of preparation (see protocol for details).


Classification: Animal Origin-Free
Concentrated: 10 X
Form: Liquid
Green Features: Sustainable packaging
Inorganic Salts: Calcium,Magnesium
Osmolality: 2500 - 3000 mOsm/kg
Phenol Red Indicator: Phenol Red
Product Line: Gibco®
Product Size: 500 mL
Shipping Condition: Room Temperature
pH Range: 5.5 - 6.0

Contents & storage

Storage conditions: 15-30° C.
Shipping conditions: Ambient.
Shelf life: 36 months from date of manufacture.