This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.
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HBSS (10X), calcium, magnesium, no phenol red

Catalog number:  14065056

Related applications:

Mammalian Cell Culture

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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation. Concentrated forms of Gibco® HBSS require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation (see protocol for details).

We offer a variety of Gibco® HBSS formulations for a range of cell culture applications.

This HBSS is modified as follows:

• Calcium• Phenol Red
• Magnesium• Sodium Bicarbonate
• Glucose

The complete formulation is available.

cGMP Manufacturing and Quality System
Gibco® HBSS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® HBSS product made in our Scotland facility (14065-049). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For In Vitro Diagnostic Use. For Research Use Only in China.


Classification: Animal Origin-Free
Form: Liquid
Inorganic Salts: Calcium, Magnesium
Osmolality: 2700 - 3070 mOsm/kg
Phenol Red Indicator: No Phenol Red
Product Line: Gibco®
Shipping Condition: Room Temperature
Sodium Pyruvate Additive: No Sodium Pyruvate
Concentration: 10 X
pH Range: 4.4 - 4.7

Contents & storage

Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture