This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.

HBSS, no calcium, no magnesium, no phenol red This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 14175103

Gibco™  Related applications: Mammalian Cell Culture

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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation.

We offer a variety of Gibco® HBSS formulations for a range of cell culture applications.

This HBSS is modified as follows:

• Glucose• Calcium
• Magnesium
• Phenol Red

The complete formulation is available.

cGMP Manufacturing and Quality System
Gibco® HBSS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® HBSS product made in our Scotland facility (14175-053). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”


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Form: Liquid
pH Range: 6.7 - 7.8
Osmolality: 270 - 295 mOsm/kg
Concentrated: 1 X
Product Size: 10 x 500 mL
Classification: Animal Origin-Free
Inorganic Salts: No Calcium, No Magnesium
Phenol Red Indicator: No Phenol Red
Sodium Pyruvate Additive: No Sodium Pyruvate
Green Features: Fewer resources used, Less waste

Contents & storage

Storage conditions: 15°C to 30°C
Shipping conditions: Room temperature
Shelf life: 36 months from date of manufacture


Manuals & protocols

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