Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.

This HBSS is modified as follows:

• With: Glucose, Phenol Red
• Without: Calcium, Magnesium, Sodium Bicarbonate

The complete formulation is available.

Concentrated forms of Gibco™ HBSS require pH adjustment and filtration at the time of preparation (see protocol for details).