Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. We offer a variety of Gibco® HBSS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.
Product Intended Use For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.
cGMP Manufacturing and Quality System Gibco® HBSS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® HBSS product made in our Scotland facility (14185-045). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Concentrated forms of Gibco® HBSS require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation (see protocol for details).
For In Vitro Diagnostic Use. For Research Use Only in China.
For Use With (Application)
Variety of Cell Culture Applications; Such as Washing Cells Before Dissociation; Transporting Cells or Tissue Samples; Diluting Cells for Counting; and Preparing Reagents
Hank's Balanced Salt
5.8 to 6.1
2700 - 3000 mOsm/kg
No Calcium, No Magnesium
Phenol Red Indicator
No Phenol Red
5.8 - 6.1
Sodium Pyruvate Additive
No Sodium Pyruvate
Contents & Storage
Storage conditions: 15-30° C Shipping conditions: Ambient Shelf life: 24 months from date of manufacture