This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.

HBSS (10X), no calcium, no magnesium, no phenol red This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number: 14185052

Gibco™  Related applications: Mammalian Cell Culture

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Description

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents. Formulations without calcium and magnesium are required for rinsing chelators from the culture before cell dissociation. We offer a variety of Gibco® HBSS formulations for a range of cell culture applications. Find the right formulation using the media selector tool.

This HBSS is modified as follows:

WithWithout
• Glucose• Calcium
• Magnesium
• Phenol Red
• Sodium Bicarbonate

The complete formulation is available.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law.

cGMP Manufacturing and Quality System
Gibco® HBSS is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco® HBSS product made in our Scotland facility (14185-045). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Concentrated forms of Gibco® HBSS require sodium bicarbonate addition, pH adjustment, and filtration at the time of preparation (see protocol for details).

“This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”

Specifications

Form: Liquid
pH Range: 5.8 - 6.1
Osmolality: 2700 - 3000 mOsm/kg
Concentrated: 10 X
Product Size: 500 mL
Classification: Animal Origin-Free
Inorganic Salts: No Calcium, No Magnesium
Phenol Red Indicator: No Phenol Red
Sodium Pyruvate Additive: No Sodium Pyruvate
Green Features: Fewer resources used, Less waste
Shipping Condition: Room Temperature

Contents & storage

Storage conditions: 15-30° C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

Documents

Manuals & protocols

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