Invitrogen™
Dynabeads™ Co-Immunoprecipitation Kit
Catalog number: 14321D
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Description
Dynabeads® Co-Immunoprecipitation Kit allows easy coupling of an antibody of your choice to the surface of uniform, 2.8 µm superparamagnetic Dynabeads® M-270 Epoxy beads. Following immobilization of your antibody, use the coupled beads and the buffers supplied in the kit for co-immunoprecipitation (co-IP) of proteins, intact protein complexes, or intact protein-nucleic acid complexes and to elute those complexes from the beads. In addition to Dynabeads®, the kit supplies the buffers needed for efficient antibody coupling, gentle washing, and elution of the co-isolated protein complexes.• Optimized buffers and Epoxy-coated Dynabeads® for efficient co-IP included in the kit
• Gentle separation technology: preserves native protein conformations and large-protein complexes
• Covalent antibody coupling to the Dynabeads® avoids co-elution of the antibody with the target proteins
Efficient, easy coupling
Antibodies are covalently coupled to Dynabeads® M-270 Epoxy beads, minimizing the risk of bound antibody contaminating your final eluate. Dynabeads® M-270 Epoxy beads exhibit ultra-low background binding and don't require blocking before use. The supplied buffers are optimized to give consistent and reliable results and the kit includes 8 different binding, washing, and elution buffers, in addition to Dynabeads® M-270 Epoxy to enable you to purify complex protein structures of any size. All kit components are analytical grade and are compatible with protease and phosphatase inhibitors. The only thing you need to supply is the antibody of choice. The protocol describes coupling, sample preparation (detergent and cryolysis methods), co-IP and elution, and is compatible with western blot/silver stain, Coomassie™ stain, mass spectrometry, or extraction of nucleic acids from isolated DNA/RNA binding protein complexes.
Magnetic bead-based separation offers easy handling
Captured proteins and protein complexes are easily separated, washed, and eluted using a DynaMag™ magnet, and the magnetic separation properties of the Dynabeads®. The kit is designed for and tested with cultured yeast and mammalian cells and is compatible with tissue, insect, bacteria, and othe lysates.
Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.
• Dynabeads® M-270 Epoxy is also available as a stand-alone product, without the buffers supplied.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
| Surface Functionality: | Epoxy |
|---|---|
| Final Product: | Protein Complexes (Intact), Protein⁄Nucleic Acid Complexes |
| Form: | Freeze Dried |
| High Throughput Compatibility: | Not High Throughput-Compatible (Manual) |
| Product Line: | Dynabeads® |
| Product Size: | 40 reactions |
| Sample Type (General): | Cells, Tissue |
| Sample Type (Specific): | Cell Cultures, Cell Extracts, Cell Lysates, Disks, Intact Proteins, Normalized Buccal Cells, Plant, Tissue Digests |
| Shipping Condition: | Room Temperature |
Contents & storage
Kit Components:
• Dynabeads® M-270 Epoxy (>60 mg freeze-dried)
• Buffer C1 (20 mL)
• Buffer C2 (8 mL)
• Buffer HB (15 mL)
• Buffer LB (15 mL)
• Buffer SB (40 mL)
• 5X IP Buffer (120 mL)
• 5X LWB (24 mL)
• Buffer EB (4 mL)
Upon receipt, store all components at 2°C to 8°C or room temperature.
• Dynabeads® M-270 Epoxy (>60 mg freeze-dried)
• Buffer C1 (20 mL)
• Buffer C2 (8 mL)
• Buffer HB (15 mL)
• Buffer LB (15 mL)
• Buffer SB (40 mL)
• 5X IP Buffer (120 mL)
• 5X LWB (24 mL)
• Buffer EB (4 mL)
Upon receipt, store all components at 2°C to 8°C or room temperature.
