Invitrogen™

Dynabeads™ Co-Immunoprecipitation Kit

Catalog number: 14321D
Invitrogen™

Dynabeads™ Co-Immunoprecipitation Kit

Catalog number: 14321D
Catalog Number
14321D
Unit Size
40 reactions
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
14321D40 reactions
-
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The Dynabeads™ Co-Immunoprecipitation Kit is intended for the coupling of an antibody to the surface of Dynabeads™ M-270 Epoxy beads. The coupled beads and supplied buffers can then be used for co-immunoprecipitation (co-IP) of proteins, intact protein complexes, or intact protein-nucleic acid complexes. In addition to epoxy-activated Dynabeads magnetic beads, the kit includes the buffers needed for efficient antibody coupling, gentle washing, and elution of the co-isolated protein complexes.

Benefits of Dynabeads Co-Immunoprecipitation Kit include:
Convenience—contains optimized buffers and epoxy-activated Dynabeads magnetic beads for efficient co-IP
Stabile—gentle separation technology preserves native protein conformations and large protein complexes
Flexibility—allows coupling of any antibody of your choice
Robustness—covalent antibody coupling to the Dynabeads magnetic beads avoids co-elution of the antibody with the target proteins

Efficient and easy coupling
With this kit, antibodies are covalently coupled to Dynabeads M-270 Epoxy beads, minimizing the risk of bound antibodies contaminating the final eluate. Dynabeads M-270 Epoxy beads exhibit ultra-low background binding and do not require blocking before use. The supplied buffers are optimized to give consistent and reliable results. The kit includes eight different binding, washing, and elution buffers, in addition to Dynabeads M-270 Epoxy beads, to enable purification of complex protein structures of any size. All kit components are analytical grade and compatible with protease and phosphatase inhibitors. Target specific antibody is required for coupling, but not included. The protocol includes coupling, sample preparation (detergent and cryolysis methods), co-IP, and elution and is compatible with western blot/silver stain, Coomassie stain, mass spectrometry, and extraction of nucleic acids from isolated DNA/RNA binding protein complexes.

Magnetic bead–based separation offers easy handling
Captured proteins and protein complexes are easily separated, washed, and eluted using a DynaMag magnet due to the magnetic properties of the Dynabeads M-270 Epoxy beads. The kit is designed for and tested with cultured yeast and mammalian cells and is compatible with tissue, insect, bacterial, and other lysates.

Automation-ready for use with KingFisher purification systems
The 2.8-μm magnetic beads are ideal for high-throughput enrichment. This process can be automated using any of our KingFisher sample purification systems

Commercial supply
Our manufacturing sites are ISO 13485–certified. Bead characteristics and manufacturing conditions help ensure consistency and batch reproducibility, making them an ideal choice for commercial supply. If you are in the process of customizing the Dynabeads Co-Immunoprecipitation Kit in a commercial product/service or need a larger bulk volume of this product, please contact us at oemdynal@lifetech.com or read more on our Dynabeads OEM page

Learn more about Dynabeads magnetic beads and KingFisher Sample Purification Systems:
Dynabeads M-270 Epoxy beads supplied in the kit can be purchased separately
See our immunoprecipitation selection table, data, and references
Dynabeads magnetic beads selection guide
See magnets for Dynabeads magnetic beads separations
KingFisher automation protocols
Watch a video about the KingFisher Flex instrument

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Certifications/Compliance
ISO9001 and ISO13485
Color
Brown
For Use With (Application)
Co-immunoprecipitation
Material
Magnetic Polystyrene
Product Type
Co-IP Kit
Final Product Type
Protein Complexes (Intact), Protein/Nucleic Acid Complexes
Format
Kit
For Use With (Equipment)
KingFisher Sample Purification System, DynaMag magnets
High-throughput Compatibility
High-throughput Compatible
No. of Tests
40 Tests
Product Line
Dynabeads™
Quantity
60 mg
Sample Type
Cell Cultures, Disks, Plant, Tissue Digests, Cell Extracts, Cell Lysates, Intact Proteins, Plant, Normalized Buccal Cells
Shelf Life
36 months from date of manufacture
Shipping Condition
Ambient Temperature
Size Distribution
CV <5%
Surface Functionality
Epoxy
Regulatory Status
For Research Use Only

Contents & Storage

• Dynabeads M-270 Epoxy (>60 mg freeze-dried)
• Buffer C1 (20 mL)
• Buffer C2 (8 mL)
• Buffer HB (15 mL)
• Buffer LB (15 mL)
• Buffer SB (40 mL)
• 5X IP Buffer (120 mL)
• 5X LWB (24 mL)
• Buffer EB (4 mL)

Store at 2°C to 8°C or room temperature.

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