Pierce™ Maleic Anhydride Activated Plates, Clear, 8-Well Strip

Catalog number: 15100

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

These Thermo Scientific Pierce Maleic Anhydride Activated 8-Well Strip Plates are clear, 96-well strip plates that allow attachment of amine-containing peptides and other molecules to microplate wells for use in binding assays.

Features of Maleic Anhydride Activated Plates:

• Spontaneously react with primary amines
• A direct, simple approach to covalently attach molecules to the well surface of an EIA plate
• Maleic anhydride maintains its integrity and availability for coupling for months under dry, room temperature conditions

These plates are useful for immobilizing peptides and other ligands that do not coat efficiently by passive adsorption. Reaction of the maleic anhydride groups with primary amines (-NH2) results in formation of amide bonds that are stable at neutral pH and above. Acidic conditions hydrolyze the bond; for example, the half-life of hydrolysis at pH 3.5 and 37°C is approximately 11 hours (Ref.1). Therefore, coating reactions are best performed at pH 8-9, and the reacted plates are best used at pH > 7 for ELISA and other methods.

Related Products
Pierce™ Maleic Anhydride Activated Plates, Clear, 96-Well
Pierce™ Maleic Anhydride Activated Plates, White, 96-Well
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Color: Clear
Format: 96-well strip plate
Plate Type: Detection Plate, ELISA, Immunoassay
Product Size: 5 plates
Plate Blocking: None
Surface Coating: Maleic Anhydride
Binding Property: Covalent, Standard Capacity
Detection Method: Colorimetric

Contents & storage

Upon receipt store plates at room temperature. Product shipped at ambient temperature.

Documents

Manuals & protocols