Pierce™ Protein L Coated Plates, Clear, 96-Well

Catalog number: 15190

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Thermo Scientific Pierce Protein L Coated Plates provide an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plate-based assay techniques.

These plates are uniformly and stably coated with Protein L, and enable antibodies or pre-established antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.

Features of the plates:

• Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption
• Orient antibodies for maximum antigen-binding capacity
• Immobilize antibodies for plate assays without prior purification
• Ensure minimal variation (<5% well-to-well) from consistent coating
• Reduce nonspecific binding because plates are pre-blocked with SuperBlock Blocking Buffer
• Bind 2 to 5 pmol of antibody per well (based on tests with rabbit IgG)

Protein L Coated Microplates:
• Binds to all classes of immunoglobulins (IgG, IgM, IgA, IgE and IgD)
• Binds to the VL region of kappa light chains (human I, III, IV and Mouse I) without interfering with antigen-binding sites
• Binds to Fab fragments and ScFv
• Does not bind bovine, goat or sheep immunoglobulin
• Binds weakly to rabbit immunoglobulin
• Pre-blocked to reduce nonspecific binding

Related Products
Pierce™ Protein A Coated Plates, Clear, 8-Well Strip
Pierce™ Protein A/G Coated Plates, Clear, 8-Well Strip
Pierce™ Protein G Coated Plates, Clear, 8-Well Strip
For Research Use Only. Not for use in diagnostic procedures.


Color: Clear
Format: 96-well plate
Plate Type: Detection Plate, ELISA, Immunoassay
Product Size: 5 plates
Plate Blocking: SuperBlock™ Buffer
Surface Coating: Protein L
Binding Property: Affinity, Standard Capacity
Detection Method: Colorimetric

Contents & storage

Note: Plates are activated to 1µL and supplied pre-blocked with SuperBlock™ Blocking Buffer. Use these plates for single-antibody assays only. In multiple antibody assays, such as sandwich ELISAs, the first antibody cannot block all binding sites and subsequent antibodies will bind to the plate, resulting in false positives.

Upon receipt store plates at 4°C in unopened pouches. Place opened, unused plates in a resealable bag and store desiccated at 4°C. Plates are shipped at ambient temperature.


Manuals & protocols