Invitrogen™

Plasmid pBR322

Catalog number:  15367014

Related applications:

Restriction Enzyme Cloning

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Description

Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !-lactamase (AmpR) gene and eight unique sites within the TetR gene. The plasmid, purified from DH10B™ E. coli, also has 14 non-selectable unique restriction endonuclease recognition sites. Unique cleavage sites for Hind III and Cla I are found in the promoter of the tetracycline resistance gene. Insertion of DNA at either of these two sites usually results in loss of tetracycline resistance.

Performance and Quality Testing: Purity, DNA structure, and selected restriction endonuclease fragmentation patterns are verified by agarose gel analysis. Transformation of E. coli HB101 to confirm AmpR and TetR is confirmed by plating on selective medium.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cloning Method: Restriction Enzyme ⁄ MCS
Product Size: 25 µg
Shipping Condition: Approved for shipment on Wet or Dry Ice
Vector Type: pBR322

Contents & storage

pBR322 is supplied at 0.25 µg/µl in 10 mM Tris-HCl (pH 7.4), 5 mM NaCl, 0.1 mM EDTA. Store at +4°C.