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Trypsin-EDTA (0.5%), no phenol red This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  15400054

Related applications:

Mammalian Cell Culture

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Gibco® Trypsin-EDTA is made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas. Due to its digestive strength, trypsin solution is widely used for cell dissociation, routine cell culture passaging, and primary tissue dissociation. Concentrated forms of trypsin can be aseptically diluted to 1X using a balanced salt solution without calcium and magnesium. The trypsin concentration required for dissociation varies with cell type and experimental requirements. See the complete range of Gibco® trypsin solutions and recommended dissociation conditions.

We offer a variety of trypsin formulations and animal origin-free TrypLE™ reagent that feature:

Quality testing
Documented traceability
Dual-site cGMP Manufacturing

This trypsin is modified as follows:

• EDTA• Phenol Red

The complete formulation is available.

Quality testing
Gibco® Trypsin solutions are tested for pH, osmolality, sterility, and performance. In addition, prior to manufacturing, the raw materials are verified for e-beam irradiation and tested for endotoxin, PPV, PCV 1/2, mycoplasma, bacterial, fungal, and viral contamination, as well as multiple activity assays, ash analysis, and moisture analysis.

Documented traceability
We can provide detailed documentation to meet your regulatory needs. Gibco® Trypsin information available includes lot traceability, animal origin certificates, lot analyses, irradiation certificates, a viral inactivation summary, and supply chain transparency.

cGMP manufacturing and quality system
Gibco® Trypsin is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
For Research Use or Further Manufacturing. Not for use in diagnostic procedures.


Chelators: EDTA
Classification: Animal Origin
Concentrated: 10 X
Form: Liquid
Green Features: Sustainable packaging
Osmolality: 300 - 345 mOsm/kg
Phenol Red Indicator: No Phenol Red
Product Line: Gibco®
Product Size: 100 mL
Reagent Type: Trypsin
Shipping Condition: Wet Ice
Tests Performed: In Vitro Bioassay
pH Range: 7.1 - 8.0

Contents & storage

Storage conditions: -5°C to -20°C
Shelf life: 18 months from date of manufacture