Thermo Scientific™

Pierce™ Nickel Coated Plates, Clear, 96-Well

Catalog number: 15442
Thermo Scientific™

Pierce™ Nickel Coated Plates, Clear, 96-Well

Catalog number: 15442
Catalog Number
15442
Unit Size
5 plates
Price (USD)
264.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
154425 plates
264.00
Your Price:
***
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Thermo Scientific Pierce Nickel Coated Clear 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These clear plates can be used with colorimetric detection methods.

Features of Pierce Nickel Coated Plates:

• Ni2+ activated surface enables metal-chelate binding of His-tagged proteins
• Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene
• Better binding for sensitive assays compared to other commercially available nickel-activated plates
• Detection limit: 1ng of polyhistidine fusion protein
• Binding capacity: approx. 9 pmol His-tagged protein (27kDa) per well
• Activation level: 200 µL

These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.

Related Products
Pierce™ Nickel Coated Plates, Clear, 8-Well Strip
Pierce™ Nickel Coated Plates, White, 96-Well
Pierce™ Nickel Coated Plates, Black, 96-Well
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Binding Property
Standard Capacity, Affinity
Format
96-well
Description
Pierce Nickel Coated Plates, Clear, 96-Well
Detection Method
Colorimetric
Packaging
5 Plate
Product Line
Pierce™
Color
Clear
Product Type
Microplate
Material
Polystyrene
Volume (Metric) Well
200 μL
No. of Wells
96
Surface Treatment
Divalent Nickel, Metal Chelate
Plate Blocking
Blocker™ BSA Buffer

Contents & Storage

Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.

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