Electrochemical sensor for multiplex biomarkers detection.
AuthorsWei F, Patel P, Liao W, Chaudhry K, Zhang L, Arellano-Garcia M, Hu S, Elashoff D, Zhou H, Shukla S, Shah F, Ho CM, Wong DT,
JournalClin Cancer Res
PubMed ID19509137
'Multiplexing assay of biomarkers at the point-of-care is an elusive goal for molecular diagnostics. Here, we report an electrochemical (EC) sensor for oral cancer detection based on the simultaneous detection of two salivary biomarkers: interleukin (IL)-8 mRNA and IL-8 protein. Under the multiplexing mode, the limit of detection of salivary ... More
Size-based separations as an important discriminator in development of proximity ligation assays for protein or organism detection.
AuthorsLeslie DC, Sohrabi A, Ikonomi P, McKee ML, Landers JP,
JournalElectrophoresis
PubMed ID20419707
'Proximity ligation is a powerful technique to measure minute concentrations of target protein with high specificity, and it has been demonstrated to be effective on a wide variety of protein targets. The proximity ligation assay (PLA) technique is shown to be compromised by the amplification of a nonspecific fluorescent product ... More
Eps homology domain endosomal transport proteins differentially localize to the neuromuscular junction.
AuthorsMate SE, Van Der Meulen JH, Arya P, Bhattacharyya S, Band H, Hoffman EP,
JournalSkelet Muscle
PubMed ID22974368
'Recycling of endosomes is important for trafficking and maintenance of proteins at the neuromuscular junction (NMJ). We have previously shown high expression of the endocytic recycling regulator Eps15 homology domain-containing (EHD)1 proteinin the Torpedo californica electric organ, a model tissue for investigating a cholinergic synapse. In this study, we investigated ... More
Unlike CD4+ T-cell help, CD28 costimulation is necessary for effective primary CD8+ T-cell influenza-specific immunity.
AuthorsSeah SG, Carrington EM, Ng WC, Belz GT, Brady JL, Sutherland RM, Hancock MS, La Gruta NL, Brown LE, Turner SJ, Zhan Y, Lew AM,
JournalEur J Immunol
PubMed ID22585421
The importance of costimulation on CD4(+) T cells has been well documented. However, primary CTLs against many infections including influenza can be generated in the absence of CD4(+) T-cell help. The role of costimulation under such ... More
Enzymatic route to preparative-scale synthesis of UDP-GlcNAc/GalNAc, their analogues and GDP-fucose.
AuthorsZhao G, Guan W, Cai L, Wang PG,
JournalNat Protoc
PubMed ID20224564
Enzymatic synthesis using glycosyltransferases is a powerful approach to building polysaccharides with high efficiency and selectivity. Sugar nucleotides are fundamental donor molecules in enzymatic glycosylation reactions by Leloir-type glycosyltransferases. The applications of these donors are restricted by their limited availability. In this protocol, N-acetylglucosamine (GlcNAc)/N-acetylgalactosamine (GalNAc) are phosphorylated by N-acetylhexosamine ... More
Increased sphingoid base-1-phosphates and failure of neural tube closure after exposure to fumonisin or FTY720.
Fumonisin B(1) (FB(1)) is a mycotoxin produced by a common fungal contaminant of corn. Ingestion of FB(1)-contaminated food is associated with increased risk for neural tube defects (NTDs). FB(1) induces NTDs in inbred LM/Bc mice. FB(1) inhibits ceramide synthase in de novo sphingolipid biosynthesis, resulting in accumulation of sphinganine and ... More
Rapid in situ codetection of noncoding RNAs and proteins in cells and formalin-fixed paraffin-embedded tissue sections without protease treatment.
Authorsde Planell-Saguer M, Rodicio MC, Mourelatos Z,
JournalNat Protoc
PubMed ID20539282
Noncoding RNAs (ncRNAs) comprise a diverse group of RNAs that function in essential cellular processes such as pre-mRNA splicing and mRNA translation and also regulate various aspects of gene expression in physiology and development. Methods of subcellular and tissue localization of ncRNAs are essential to understand their biological roles and ... More