UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Catalog number:  15593031

 Related applications:

DNA Extraction

| RNA Extraction

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UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.

Using Phenol:Chloroform:Isoamyl Alcohol
When mixtures are extracted with Phenol:Chloroform:Isoamyl Alcohol, proteins are denatured and collected in the organic phase or at the interphase, while nucleic acids remain in the aqueous phase. UltraPure™ Phenol:Chloroform:lsoamyl Alcohol contains no preservatives. It is packaged under an inert gas in shatter-resistant, plastic-coated amber bottles.

Performance and quality testing
The appearance of the solution is evaluated at room temperature. No RNase or DNase activity is detected.
WARNING: Cancer and Reproductive Harm –
For Research Use Only. Not for use in diagnostic procedures.


Prop-65 Warning: Control Code 67
Chemical Name: Phenols
Form: Liquid
Product Line: UltraPure™
Product Size: 100 mL
Purity or Quality Grade: Molecular Biology Grade
pH: pH 8.05
Shipping Condition: Room Temperature

Contents & storage

Store at 2°C to 8°C. To extend product stability, it is recommended that the gas volume above the solution be flushed with argon or nitrogen following each use.


Manuals & protocols