UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v)

Catalog number:  15593031

 Related applications: DNA Extraction | RNA Extraction

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UltraPure™ Phenol:Chloroform:Isoamyl Alcohol (25:24:1, v/v) is used in the purification of nucleic acids. This reagent consists of highly pure chloroform, isoamyl alcohol, and UltraPure™ Phenol saturated with Tris-HCl.

Using Phenol:Chloroform:Isoamyl Alcohol
When mixtures are extracted with Phenol:Chloroform:Isoamyl Alcohol, proteins are denatured and collected in the organic phase or at the interphase, while nucleic acids remain in the aqueous phase. UltraPure™ Phenol:Chloroform:lsoamyl Alcohol contains no preservatives. It is packaged under an inert gas in shatter-resistant, plastic-coated amber bottles.

Performance and quality testing
The appearance of the solution is evaluated at room temperature. No RNase or DNase activity is detected.
For Research Use Only. Not for use in diagnostic procedures.


Chemical Name: Phenols
Form: Liquid
Product Line: UltraPure™
Product Size: 100 mL
Purity or Quality Grade: Molecular Biology Grade
pH: pH 8.05
Shipping Condition: Room Temperature

Contents & storage

Store at 2°C to 8°C. To extend product stability, it is recommended that the gas volume above the solution be flushed with argon or nitrogen following each use.


Manuals & protocols

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