UltraPure™ Phenol:Water (3.75:1, v/v) is used in the purification of nucleic acids. This liquid reagent consists of UltraPure™ Phenol and highly pure deionized water. The reagent can be used to prepare different types of buffer-saturated phenol. When mixtures are extracted with buffer-saturated phenol, proteins are denatured and collect in the organic phase or at the interphase, while nucleic acids remain in the aqueous phase. UltraPure™ Phenol:Water (3.75:1, v/v) contains no preservatives. It is packaged under an inert gas in shatter-resistant, plastic-coated amber bottles.

Performance and Quality Testing: The appearance of the solution is evaluated at room temperature. No RNase or DNase activity detected.