Recovery of an arenavirus entirely from RNA polymerase I/II-driven cDNA.
AuthorsFlatz L,Bergthaler A,de la Torre JC,Pinschewer DD
JournalProceedings of the National Academy of Sciences of the United States of America
PubMed ID16537369
The prototypic arenavirus lymphocytic choriomeningitis virus has been a primary workhorse of viral immunologists for almost a century, and it has served as an important model for studying basic principles of arenavirus molecular biology. Its negative-stranded bisegmented RNA genome has, however, posed a major obstacle to attempts at manipulating the ... More
Authors
Journal
PubMed ID16195417
Hyaluronan-CD44-ERK1/2 regulate human aortic smooth muscle cell motility during aging.
ClC-3 is a fundamental molecular component of volume-sensitive outwardly rectifying Cl- channels and volume regulation in HeLa cells and Xenopus laevis oocytes.
AuthorsHermoso M, Satterwhite CM, Andrade YN, Hidalgo J, Wilson SM, Horowitz B, Hume JR
JournalJ Biol Chem
PubMed ID12183454
Volume-sensitive osmolyte and anion channels (VSOACs) are activated upon cell swelling in most vertebrate cells. Native VSOACs are believed to be a major pathway for regulatory volume decrease (RVD) through efflux of chloride and organic osmolytes. ClC-3 has been proposed to encode native VSOACs in Xenopus laevis oocytes and in ... More
A novel single amino acid deletion caspase-8 mutant in cancer cells that lost proapoptotic activity.
AuthorsLiu B, Peng D, Lu Y, Jin W, Fan Z.
JournalJ Biol Chem
PubMed ID12055196
'Caspase-8 is an important initiation caspase that activates the caspase cascade during death receptor-mediated apoptosis. We here report a novel caspase-8 mutant with a naturally occurring deletion of leucine 62 (Delta Leu62casp-8). Delta Leu62casp-8 has a shorter half-life than its wild-type counterpart. Unlike wild-type caspase-8, Delta Leu62casp-8 failed to interact ... More
Gata factor translation is the final downstream step in the amino acid/tor-mediated vitellogenin gene expression in the anautogenous mosquito aedes aegypti.
'Ingestion of blood is required for vector mosquitoes to initiate reproductive cycles determining their role as vectors of devastating human diseases. Nutritional signaling plays a pivotal role in regulating mosquito reproduction. Transcription of yolk protein precursor genes is repressed until mosquitoes take blood. Previously, we have shown that to signal ... More
RasGRP4, a new mast cell-restricted Ras guanine nucleotide-releasing protein with calcium- and diacylglycerol-binding motifs. Identification of defective variants of this signaling protein in asthma, mastocytosis, and mast cell leukemia patients and demonstration of the importance of RasGRP4 in mast cell development and function.
Authors Yang Yi; Li Lixin; Wong Guang W; Krilis Steven A; Madhusudhan M S; Sali Andrej; Stevens Richard L;
JournalJ Biol Chem
PubMed ID11956218
'A cDNA was isolated from interleukin 3-developed, mouse bone marrow-derived mast cells (MCs) that contained an insert (designated mRasGRP4) that had not been identified in any species at the gene, mRNA, or protein level. By using a homology-based cloning approach, the approximately 2.6-kb hRasGRP4 transcript was also isolated from the ... More
Reconstitution of human DNA polymerase delta using recombinant baculoviruses: the p12 subunit potentiates DNA polymerizing activity of the four-subunit enzyme.
Authors Podust Vladimir N; Chang Long-Sheng; Ott Robert; Dianov Grigory L; Fanning Ellen;
JournalJ Biol Chem
PubMed ID11711545
'Eukaryotic DNA polymerase delta is thought to consist of three (budding yeast) or four subunits (fission yeast, mammals). Four human genes encoding polypeptides p125, p50, p66, and p12 have been assigned as subunits of DNA polymerase delta. However, rigorous purification of human or bovine DNA polymerase delta from natural sources ... More
Functional linkage between the endoplasmic reticulum protein Hsp47 and procollagen expression in human vascular smooth muscle cells.
AuthorsRocnik EF, van der Veer E, Cao H, Hegele RA, Pickering JG
JournalJ Biol Chem
PubMed ID12163502
'Hsp47 is a heat stress protein that interacts with procollagen in the lumen of the endoplasmic reticulum, which is vital for collagen elaboration and embryonic viability. The precise actions of Hsp47 remain unclear, however. To evaluate the effects of Hsp47 on collagen production we infected human vascular smooth muscle cells ... More
Norepinephrine increases I kappa B alpha expression in astrocytes.
'The neurotransmitter norepinephrine (NE) can inhibit inflammatory gene expression in glial cells; however, the mechanisms involved are not clear. In primary astrocytes, NE dose-dependently increased the expression of inhibitory I kappa B alpha protein accompanied by an increase in steady state levels of I kappa B alpha mRNA. Maximal increases ... More
Oxytocin induces differentiation of P19 embryonic stem cells to cardiomyocytes.
Authors Paquin Joanne; Danalache Bogdan A; Jankowski Marek; McCann Samuel M; Gutkowska Jolanta;
JournalProc Natl Acad Sci U S A
PubMed ID12093924
'We recently discovered the existence of the oxytocin/oxytocin receptor (OT/OTR) system in the heart. Activation of cardiac OTR stimulates the release of atrial natriuretic peptide (ANP), which is involved in regulation of blood pressure and cell growth. Having observed elevated OT levels in the fetal and newborn heart at a ... More
Regulation of the Farnesoid X Receptor (FXR) by Bile Acid Flux in Rabbits.
Authors Xu Guorong; Pan Lu-Xing; Li Hai; Forman Barry M.; Erickson Sandra K.; Shefer Sarah; Bollineni Jaya; Batta Ashok K.; Christie Jennifer; Wang Tsu-Hong; Michel John; Yang Steve; Tsai Richard; Lai Lily; Shimada Kohei; Tint G. Stephen; Salen Gerald;
JournalJ Biol Chem
PubMed ID12401785
'We investigated the roles of hydrophobic deoxycholic acid (DCA) and hydrophilic ursocholic acid (UCA) in the regulation of the orphan nuclear farnesoid X receptor (FXR) in vivo. Rabbits with bile fistula drainage (removal of the endogenous bile acid pool), rabbits with bile fistula drainage and replacement with either DCA or ... More
Molecular and functional analysis of the lepB gene, encoding a type I signal peptidase from Rickettsia rickettsii and Rickettsia typhi.
AuthorsRahman MS, Simser JA, Macaluso KR, Azad AF,
JournalJ Bacteriol
PubMed ID12867468
'The type I signal peptidase lepB genes from Rickettsia rickettsii and Rickettsia typhi, the etiologic agents of Rocky Mountain spotted fever and murine typhus, respectively, were cloned and characterized. Sequence analysis of the cloned lepB genes from R. rickettsii and R. typhi shows open reading frames of 801 and 795 ... More
Expression profiling and gene discovery in the mouse lens.
AuthorsWride MA, Mansergh FC, Adams S, Everitt R, Minnema SE, Rancourt DE, Evans MJ,
JournalMol Vis
PubMed ID12942050
'PURPOSE: Defects in the development and physiology of the lens can result in cataracts (opacification of the lens), which are currently treatable only by surgical removal. The lens is also an excellent system for understanding fundamental biological processes such as cellular differentiation and ageing. Here, microarrays have been used to ... More
Functional analysis of the rat N-methyl-D-aspartate receptor 2A promoter: multiple transcription starts points, positive regulation by Sp factors, and translational regulation.
AuthorsLiu A, Zhuang Z, Hoffman PW, Bai G,
JournalJ Biol Chem
PubMed ID12746457
'N-Methyl-d-aspartate (NMDA) receptor subunit 2A (NR2A) is an important modulatory component of the NMDA subtype of glutamate receptors. To investigate the transcription mechanism of the NR2A gene, we cloned the 5''-flanking sequence from a rat genomic library. RNA mapping with rat brain RNA revealed two sets of major and several ... More
The transcriptome profile of human embryonic stem cells as defined by SAGE.
AuthorsRichards M, Tan SP, Tan JH, Chan WK, Bongso A,
JournalStem Cells
PubMed ID14688391
'Human embryonic stem (ES) cell lines that have the ability to self-renew and differentiate into specific cell types have been established. The molecular mechanisms for self-renewal and differentiation, however, are poorly understood. We determined the transcriptome profiles for two proprietary human ES cell lines (HES3 and HES4, ES Cell International), ... More
Bcl-X(L) affects Ca(2+) homeostasis by altering expression of inositol 1,4,5-trisphosphate receptors.
Authors Li Chi; Fox Casey J; Master Stephen R; Bindokas Vytautas P; Chodosh Lewis A; Thompson Craig B;
JournalProc Natl Acad Sci U S A
PubMed ID12118121
'An oligonucleotide-based microarray analysis of 9,500 genes and expressed sequence tags (ESTs) demonstrated that the type 1 inositol 1,4,5-trisphosphate receptor (IP(3)R) was significantly down-regulated in Bcl-X(L)-expressing as compared with control cells. This result was confirmed at the mRNA and protein levels by Northern and Western blot analyses of two independent ... More
Liver X receptors as insulin-mediating factors in fatty acid and cholesterol biosynthesis.
Authors Tobin Kari Anne Risan; Ulven Stine M; Schuster Gertrud U; Steineger Hilde Hermansen; Andresen Sissel Mahle; Gustafsson Jan-Ake; Nebb Hilde Irene;
JournalJ Biol Chem
PubMed ID11781314
'The nuclear receptor liver X receptor (LXR) alpha, an important regulator of cholesterol and bile acid metabolism, was analyzed after insulin stimulation in liver in vitro and in vivo. A time- and dose-dependent increase in LXRalpha steady-state mRNA level was seen after insulin stimulation of primary rat hepatocytes in culture. ... More
Gene expression comparison of biopsies from Duchenne muscular dystrophy (DMD) and normal skeletal muscle.
Authors Haslett Judith N.; Sanoudou Despina; Kho Alvin T.; Bennett Richard R.; Greenberg Steven A.; Kohane Isaac S.; Beggs Alan H.; Kunkel Louis M.;
JournalProc Natl Acad Sci U S A
PubMed ID12415109
'The primary cause of Duchenne muscular dystrophy (DMD) is a mutation in the dystrophin gene leading to the absence of the corresponding RNA transcript and protein. Absence of dystrophin leads to disruption of the dystrophin-associated protein complex and substantial changes in skeletal muscle pathology. Although the histological pathology of dystrophic ... More
Cardiac troponin T variants produced by aberrant splicing of multiple exons in animals with high instances of dilated cardiomyopathy.
Authors Biesiadecki Brandon J; Elder Benjamin D; Yu Zhi-Bin; Jin Jian-Ping;
JournalJ Biol Chem
PubMed ID12377784
'Adult cardiac muscle normally expresses a single cardiac troponin T (cTnT). As a potential pathogenic mechanism for turkey dilated cardiomyopathy, the splice-out of a normally constitutive exon generates an additional low molecular weight cTnT with altered conformation and function. We further found that aberrant splicing of cTnT also occurs in ... More
A role for ASIC3 in the modulation of high-intensity pain stimuli.
Authors Chen Chih-Cheng; Zimmer Anne; Sun Wei-Hsin; Hall Jennifer; Brownstein Michael J; Zimmer Andreas;
JournalProc Natl Acad Sci U S A
PubMed ID12060708
'Acid-sensing ion channel 3 (ASIC3), a proton-gated ion channel of the degenerins/epithelial sodium channel (DEG/ENaC) receptor family is expressed predominantly in sensory neurons including nociceptive neurons responding to protons. To study the role of ASIC3 in pain signaling, we generated ASIC3 knockout mice. Mutant animals were healthy and responded normally ... More
The Chapel Hill hemophilia A dog colony exhibits a factor VIII gene inversion.
AuthorsLozier Jay N.; Dutra Amalia; Pak Evgenia; Zhou Nan; Zheng Zhili; Nichols Timothy C.; Bellinger Dwight A.; Read Marjorie; Morgan Richard A.;
JournalProc. Natl. Acad. Sci. U S A.
PubMed ID12242334
'In the Chapel Hill colony of factor VIII-deficient dogs, abnormal sequence (ch8, for canine hemophilia 8, GenBank no. ) follows exons 1-22 in the factor VIII transcript in place of exons 23-26. The canine hemophilia 8 locus (ch8) sequence was found in a 140-kb normal dog genomic DNA bacterial artificial ... More
Expression of the suppressor of cytokine signaling-5 (SOCS5) negatively regulates IL-4-dependent STAT6 activation and Th2 differentiation.
'The development of helper T (Th) cell subsets, which secrete distinct cytokines, plays an important role in determining the type of immune response. The IL-4-mediated Janus kinase-signal transducer and activator of transcription signaling pathway is crucial for mediating Th2 cell development. Notably, this pathway is selectively impaired in Th1 cells, ... More
Defective ureagenesis in mice carrying a liver-specific disruption of hepatocyte nuclear factor 4alpha (HNF4alpha ). HNF4alpha regulates ornithine transcarbamylase in vivo.
Authors Inoue Yusuke; Hayhurst Graham P; Inoue Junko; Mori Masataka; Gonzalez Frank J;
JournalJ Biol Chem
PubMed ID11994307
'Hepatocyte nuclear factor 4alpha (HNF4alpha) regulates the expression of many genes preferentially expressed in liver. HNF4alpha-null mice die during embryogenesis precluding the analysis of its function in the adult. To circumvent this problem, liver-specific HNF4alpha-null mice were produced. Mice lacking hepatic HNF4alpha expression exhibited increased serum ammonia and reduced serum ... More
Elevated mitochondrial reactive oxygen species generation affects the immune response via hypoxia-inducible factor-1alpha in long-lived Mclk1+/- mouse mutants.
AuthorsWang D, Malo D, Hekimi S,
JournalJ Immunol
PubMed ID20007531
'Mitochondrial reactive oxygen species (ROS) are believed to stabilize hypoxia-inducible factor (HIF)-1alpha, a transcriptional regulator of the immune response. Mclk1 encodes a mitochondrial protein that is necessary for ubiquinone biosynthesis. Heterozygote Mclk1(+/-) mutant mice are long-lived despite increased mitochondrial ROS and decreased energy metabolism. In this study, Mclk1(+/-) mutant mice ... More
Selective loss of sphingosine 1-phosphate signaling with no obvious phenotypic abnormality in mice lacking its G protein-coupled receptor, LP(B3)/EDG-3.
Authors Ishii I; Friedman B; Ye X; Kawamura S; McGiffert C; Contos J J; Kingsbury M A; Zhang G; Heller Brown J; Chun J;
JournalJ Biol Chem
PubMed ID11443127
'Sphingosine 1-phosphate (S1P) exerts diverse physiological actions by activating its cognate G protein-coupled receptors. Five S1P receptors have been identified in mammals: LP(B1)/EDG-1, LP(B2)/H218/AGR16/EDG-5, LP(B3)/EDG-3, LP(B4)/NRG-1/EDG-8, and LP(C1)/EDG-6. One of these receptors, LP(B1), has recently been shown to be essential for mouse embryonic development. Here we disrupted the lp(B3) gene ... More
Calcium regulation of matrix metalloproteinase-mediated migration in oral squamous cell carcinoma cells.
AuthorsMunshi HG, Wu YI, Ariztia EV, Stack MS,
JournalJ Biol Chem
PubMed ID12194986
'Activation of matrix metalloproteinase 2 (MMP-2) has been shown to play a significant role in the behavior of cancer cells, affecting both migration and invasion. The activation process requires multimolecular complex formation involving pro-MMP-2, membrane type 1-MMP (MT1-MMP), and tissue inhibitor of metalloproteinases-2 (TIMP-2). Because calcium is an important regulator ... More
GC box-binding transcription factors control the neuronal specific transcription of the cyclin-dependent kinase 5 regulator p35.
Authors Ross Sarah; Tienhaara Anri; Lee Ming-Sum; Tsai Li-Huei; Gill Grace;
JournalJ Biol Chem
PubMed ID11724806
'Cyclin-dependent kinase 5 (cdk5)/p35 kinase activity is highest in post-mitotic neurons of the central nervous system and is critical for development and function of the brain. The neuronal specific activity of the cdk5/p35 kinase is achieved through the regulated expression of p35 mRNA. We have identified a small 200-bp fragment ... More
Increased neonatal mortality in mice lacking cellular retinol-binding protein II.
AuthorsE X, Zhang L, Lu J, Tso P, Blaner WS, Levin MS, Li E,
JournalJ Biol Chem
PubMed ID12138113
'Cellular retinol-binding protein II (CRBP II) is a member of the cellular retinol-binding protein family, which is expressed primarily in the small intestine. To investigate the physiological role of CRBP II, the gene encoding CRBP II was inactivated. The saturable component of intestinal retinol uptake is impaired in CRBP II(-/-) ... More
Nongenomic testosterone calcium signaling. Genotropic actions in androgen receptor-free macrophages.
AuthorsGuo Z, Benten WP, Krucken J, Wunderlich F
JournalJ Biol Chem
PubMed ID12048191
'Steroid hormones exert genotropic actions through members of the nuclear receptor family. Here, we have demonstrated genotropic actions of testosterone that are independent of intracellular androgen receptors (iAR). Through plasma membrane androgen receptors (mAR), testosterone induces a rapid rise in the intracellular free Ca(2+) concentration of iAR-free murine RAW 264.7 ... More
Megakaryoblastic leukemia factor-1 transduces cytoskeletal signals and induces smooth muscle cell differentiation from undifferentiated embryonic stem cells.
AuthorsDu KL, Chen M, Li J, Lepore JJ, Mericko P, Parmacek MS,
JournalJ Biol Chem
PubMed ID14970199
'The SAP domain transcription factor myocardin plays a critical role in the transcriptional program regulating smooth muscle cell differentiation. In this report, we describe the capacity of myocardin to physically associate with megakaryoblastic leukemia factor-1 (MKL1) and characterize the function of MKL1 in smooth muscle cells (SMCs). The MKL1 gene ... More
Dual roles of modulatory calcineurin-interacting protein 1 in cardiac hypertrophy.
AuthorsVega RB, Rothermel BA, Weinheimer CJ, Kovacs A, Naseem RH, Bassel-Duby R, Williams RS, Olson EN,
JournalProc Natl Acad Sci U S A
PubMed ID12515860
'The calcium/calmodulin-dependent protein phosphatase calcineurin stimulates cardiac hypertrophy in response to numerous stimuli. Calcineurin activity is suppressed by association with modulatory calcineurin-interacting protein (MCIP)1DSCR1, which is up-regulated by calcineurin signaling and has been proposed to function in a negative feedback loop to modulate calcineurin activity. To investigate the involvement of ... More
Comparison of automated and manual nucleic Acid extraction methods for detection of enterovirus RNA.
AuthorsKnepp JH, Geahr MA, Forman MS, Valsamakis A,
JournalJ Clin Microbiol
PubMed ID12904351
'Automated nucleic acid extraction is an attractive alternative to labor-intensive manual methods. We compared two automated methods, the BioRobot M48 instrument (Qiagen, Inc.) and MagNA Pure (Roche Applied Sciences) methods, to two manual methods, the QIAamp Viral RNA Mini kit (Qiagen) and TRIzol (Invitrogen), for the extraction of enterovirus RNA. ... More
Posttranscriptional control of the expression and function of diacylglycerol acyltransferase-1 in mouse adipocytes.
Authors Yu Yi-Hao; Zhang Yiying; Oelkers Peter; Sturley Stephen L; Rader Daniel J; Ginsberg Henry N;
JournalJ Biol Chem
PubMed ID12407108
'Acyl-CoA:diacylglycerol acyltransferase-1 (DGAT1) catalyzes the final step of triglyceride synthesis in mammalian cells. Data obtained from DGAT1-knockout mice have indicated that this enzyme plays an important role in energy homeostasis. We investigated the regulation of the expression and function of DGAT1 in mouse 3T3-L1 cell as a model for mammalian ... More
Polyamines enhance synthesis of the RNA polymerase sigma 38 subunit by suppression of an amber termination codon in the open reading frame.
AuthorsYoshida M, Kashiwagi K, Kawai G, Ishihama A, Igarashi K,
JournalJ Biol Chem
PubMed ID12147703
'The mechanisms by which polyamines stimulate synthesis of the RNA polymerase sigma(38) subunit in Escherichia coli were studied. Polyamine stimulation was observed only in strains in which the 33rd codon of RpoS mRNA is a UAG termination codon instead of a CAG codon for glutamine in wild-type E. coli. Readthrough ... More
Human benign prostatic hyperplasia stromal cells as inducers and targets of chronic immuno-mediated inflammation.
AuthorsPenna G, Fibbi B, Amuchastegui S, Cossetti C, Aquilano F, Laverny G, Gacci M, Crescioli C, Maggi M, Adorini L,
JournalJ Immunol
PubMed ID19299703
'Benign prostatic hyperplasia (BPH), a highly prevalent prostatic condition, could involve an inflammatory component in disease pathogenesis. In this study, we show that human stromal prostate cells obtained from BPH tissue can actively contribute to the inflammatory process by secreting proinflammatory cytokines as well as chemokines able to recruit lymphomonuclear ... More
Characterization of three alternatively spliced isoforms of the Rel/NF-kappa B transcription factor Relish from the mosquito Aedes aegypti.
Authors Shin Sang Woon; Kokoza Vladimir; Ahmed Abduelaziz; Raikhel Alexander S;
JournalProc Natl Acad Sci U S A
PubMed ID12119421
'The Rel/NF-kappa B transcription factor Relish performs a central role in the acute-phase response to microbial challenge by activating immune antibacterial peptides. We cloned and molecularly characterized the gene homologous to Drosophila Relish from the mosquito Aedes aegypti. Unlike Drosophila Relish, Aedes Relish has three alternatively spliced transcripts encoding different ... More
Microdissection RT-PCR analysis of gene expression in pathologically defined frozen tissue sections.
Authors Hiller T; Snell L; Watson P H;
JournalBiotechniques
PubMed ID8816232
'The researchers use Trizol reagent (15596018) to isolate DNA from paraffin embedded tumor sections. 4 ug of total RNA was isolated per square cm (20um thick tumor sections). These investigators claim that 0.25 square cm sections of tumor routinely yield ~1 ug total RNA. In addition, they address ... More
A crucial role for the putative Arabidopsis topoisomerase VI in plant growth and development.
'Plant steroid hormones, brassinosteroids (BRs), play important roles throughout plant growth and development. Plants defective in BR biosynthesis or perception display cell elongation defects and severe dwarfism. Two dwarf mutants named bin3 and bin5 with identical phenotypes to each other display some characteristics of BR mutants and are partially insensitive ... More
Characterization of the SECIS binding protein 2 complex required for the co-translational insertion of selenocysteine in mammals.
AuthorsKinzy SA, Caban K, Copeland PR,
JournalNucleic Acids Res
PubMed ID16155186
'Selenocysteine is incorporated into at least 25 human proteins by a complex mechanism that is a unique modification of canonical translation elongation. Selenocysteine incorporation requires the concerted action of a kink-turn structural RNA (SECIS) element in the 3'' untranslated region of each selenoprotein mRNA, a selenocysteine-specific translation elongation factor (eEFSec) ... More
The human acid alpha-glucosidase gene is a novel target of the Notch-1/Hes-1 signaling pathway.
AuthorsYan B, Raben N, Plotz P.
JournalJ Biol Chem
PubMed ID12065598
'Acid alpha-glucosidase (GAA) is a lysosomal enzyme that degrades glycogen. A deficiency of GAA is responsible for a recessively inherited myopathy and cardiomyopathy, glycogenosis type II. Previously, we identified an intronic repressor element in the GAA gene and demonstrated that Hes-1, a basic helix-loop-helix factor, binds to a C class ... More
Estradiol differentially regulates lipocalin-type prostaglandin D synthase transcript levels in the rodent brain: Evidence from high-density oligonucleotide arrays and in situ hybridization.
AuthorsMong JA, Devidze N, Frail DE, O'Connor LT, Samuel M, Choleris E, Ogawa S, Pfaff DW,
JournalProc Natl Acad Sci U S A
PubMed ID12518068
'Microarrays comprise an efficient approach to discovering large numbers of differentially expressed mRNA transcripts in the CNS resulting from changes in hormonal milieu. We used high-density oligonucleotide microarrays to examine the short- and long-term actions of estradiol (E(2)) on the transcriptomes from the medial basal hypothalamus and other brain regions ... More
Identification of connective tissue growth factor as a target of WT1 transcriptional regulation.
AuthorsStanhope-Baker P, Williams BR,
JournalJ Biol Chem
PubMed ID10973960
'The Wilms tumor suppressor WT1 has transcription-activating and -suppressing capabilities. WT1-responsive promoters have been described; however, in large part, it remains unclear which potential downstream genes are physiologically relevant and mediate the function of WT1 in tumorigenesis and development. To identify genes regulated by WT1 in vivo, we used a ... More
Antibody mediated in vivo delivery of small interfering RNAs via cell-surface receptors.
AuthorsSong E, Zhu P, Lee SK, Chowdhury D, Kussman S, Dykxhoorn DM, Feng Y, Palliser D, Weiner DB, Shankar P, Marasco WA, Lieberman J,
JournalNat Biotechnol
PubMed ID15908939
'Delivery of small interfering RNAs (siRNAs) into cells is a key obstacle to their therapeutic application. We designed a protamine-antibody fusion protein to deliver siRNA to HIV-infected or envelope-transfected cells. The fusion protein (F105-P) was designed with the protamine coding sequence linked to the C terminus of the heavy chain ... More
A CDC45 homolog in Arabidopsis is essential for meiosis, as shown by RNA interference-induced gene silencing.
'CDC45 is required for the initiation of DNA replication in yeast and cell proliferation in mammals and functions as a DNA polymerase alpha loading factor in Xenopus. We have cloned a CDC45 homolog from Arabidopsis whose expression is upregulated at the G1/S transition and in young meiotic flower buds. One-third ... More
AuthorsChevallier J, Chamoun Z, Jiang G, Prestwich G, Sakai N, Matile S, Parton RG, Gruenberg J,
JournalJ Biol Chem
PubMed ID18644787
'Most cell types acquire cholesterol by endocytosis of circulating low density lipoprotein, but little is known about the mechanisms of intra-endosomal cholesterol transport and about the primary cause of its aberrant accumulation in the cholesterol storage disorder Niemann-Pick type C (NPC). Here we report that lysobisphosphatidic acid (LBPA), an unconventional ... More
Structural requirements for pre-microRNA binding and nuclear export by Exportin 5.
AuthorsZeng Y, Cullen BR,
JournalNucleic Acids Res
PubMed ID15356295
'The biogenesis and function of mature human microRNAs is dependent on the nuclear export of pre-microRNA precursors by Exportin 5 (Exp5). The precursor for the human miR-30 microRNA, which is a 63 nt long RNA hairpin bearing a 2 nt 3'' overhang, forms a specific complex with Exp5 and the ... More
Functional inactivation of the mouse nucleolar protein Bop1 inhibits multiple steps in pre-rRNA processing and blocks cell cycle progression.
AuthorsStrezoska Z, Pestov DG, Lau LF.
JournalJ Biol Chem
PubMed ID12048210
'Bop1 is a conserved nucleolar protein involved in rRNA processing and ribosome assembly in eukaryotes. Expression of its dominant-negative mutant Bop1 Delta in mouse cells blocks rRNA maturation and synthesis of large ribosomal subunits and induces a reversible, p53-dependent cell cycle arrest. In this study, we have conducted a deletion ... More
Identification and Characterization of Presenilin-independent Notch Signaling.
Authors Berechid Bridget E.; Kitzmann Magali; Foltz Daniel R.; Roach Arthur H.; Seiffert Dietmar; Thompson Lorin A.; Olson Richard E.; Bernstein Alan; Donoviel Dorit B.; Nye Jeffrey S.;
JournalJ Biol Chem
PubMed ID11756438
'Presenilin (PS) proteins control the proteolytic cleavage that precedes nuclear access of the Notch intracellular domain. Here we observe that a partial activation of the HES1 promoter can be detected in PS1/PS2 (PS1/2) double null cells using Notch1DeltaE constructs or following Delta1 stimulation, despite an apparent abolition of the production ... More
Authors Pinkoski Michael J; Droin Nathalie M; Green Douglas R;
JournalJ Biol Chem
PubMed ID12196549
'Members of the tumor necrosis factor (TNF) and TNF receptor families play important roles in inducing apoptosis and mediating the inflammatory response. Activated T lymphocytes can trigger the expression of Fas-ligand on non-lymphoid tissue, such as intestinal epithelial cells (IEC), and this, in turn, can induce apoptosis in the T ... More
Fibrin inhibits peripheral nerve remyelination by regulating schwann cell differentiation.
'Remyelination is a critical step for functional nerve regeneration. Here we show that fibrin deposition in the peripheral nervous system after injury is a key regulator of remyelination. After sciatic nerve crush, fibrin is deposited and its clearance correlates with remyelination. Fibrin induces phosphorylation of ERK1/2 and production of p75 ... More
Molecular basis defining human Chlamydia trachomatis tissue tropism. A possible role for tryptophan synthase.
Authors Fehlner-Gardiner Christine; Roshick Christine; Carlson John H; Hughes Scott; Belland Robert J; Caldwell Harlan D; McClarty Grant;
JournalJ Biol Chem
PubMed ID12011099
Here we report the cloning and sequencing of a region of the chlamydiae chromosome termed the
Complex DNA melting profiles of small PCR products revealed using SYBR Green I.
AuthorsLi W, Xi B, Yang W, Hawkins M, Schubart UK,
JournalBiotechniques
PubMed ID14579734
None
Association of calnexin with mutant peripheral myelin protein-22 ex vivo: a basis for
Authors Dickson K M; Bergeron J J M; Shames I; Colby J; Nguyen D T; Chevet E; Thomas D Y; Snipes G J;
JournalProc Natl Acad Sci U S A
PubMed ID12119418
Schwann cell-derived peripheral myelin protein-22 (PMP-22) when mutated or overexpressed causes heritable neuropathies with a previously unexplained
Complement C5b-9 membrane attack complex increases expression of endoplasmic reticulum stress proteins in glomerular epithelial cells.
AuthorsCybulsky AV, Takano T, Papillon J, Khadir A, Liu J, Peng H,
JournalJ Biol Chem
PubMed ID12191998
In the passive Heymann nephritis (PHN) model of membranous nephropathy, complement C5b-9 induces glomerular epithelial cell (GEC) injury, proteinuria, and activation of cytosolic phospholipase A(2) (cPLA(2)). This study addresses the role of endoplasmic reticulum (ER) stress proteins (bip, grp94) in GEC injury. GEC that overexpress cPLA(2) (produced by transfection) and ... More
Improved full-length cDNA production based on RNA tagging by T4 DNA ligase.
AuthorsClepet C, Le Clainche I, Caboche M,
JournalNucleic Acids Res
PubMed ID14704363
Second-strand cDNA priming is a central problem for full-length characterization of transcripts. A new strategy using bacteriophage T4 DNA ligase and partially degenerate adapters is proposed for grafting a sequence tag to the end of polyribonucleotides. Based on this RNA tagging system and previously described protocols, a new method for ... More
Negative autoregulation of BCL-6 is bypassed by genetic alterations in diffuse large B cell lymphomas.
Authors Wang Xing; Li Zhiping; Naganuma Akira; Ye B Hilda;
JournalProc Natl Acad Sci U S A
PubMed ID12407182
Thirty to forty percent of diffuse large B cell lymphomas (DLBCL) carry BCL-6 translocations that disrupt its 5' regulatory region. This same region is also subject to somatic hypermutations, although only a small fraction of these mutations have a detectable effect on transcription. Here, we show that transcription of the ... More
Alpha-fetoprotein, the major fetal serum protein, is not essential for embryonic development but is required for female fertility.
Authors Gabant Philippe; Forrester Lesley; Nichols Jennifer; Van Reeth Thierry; De Mees Christelle; Pajack Bernard; Watt Alistair; Smitz Johan; Alexandre Henri; Szpirer Claude; Szpirer Josiane;
JournalProc Natl Acad Sci U S A
PubMed ID12297623
The alpha-fetoprotein gene (Afp) is a member of a multigenic family that comprises the related genes encoding albumin, alpha-albumin, and vitamin D binding protein. The biological role of this major embryonic serum protein is unknown although numerous speculations have been made. We have used gene targeting to show that AFP ... More
Visualization and identification of IL-7 producing cells in reporter mice.
AuthorsMazzucchelli RI, Warming S, Lawrence SM, Ishii M, Abshari M, Washington AV, Feigenbaum L, Warner AC, Sims DJ, Li WQ, Hixon JA, Gray DH, Rich BE, Morrow M, Anver MR, Cherry J, Naf D, Sternberg LR, McVicar DW, Farr AG, Germain RN, Rogers K, Jenkins NA, Copeland NG, Durum SK,
JournalPLoS One
PubMed ID19907640
Interleukin-7 (IL-7) is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome (BAC) transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide and ECFP (enhanced cyan fluorescent protein) accumulated inside ... More
R9AP, a membrane anchor for the photoreceptor GTPase accelerating protein, RGS9-1.
Authors Hu Guang; Wensel Theodore G;
JournalProc Natl Acad Sci U S A
PubMed ID12119397
The regulator of G protein signaling (RGS)-9-1.G(beta 5) complex forms the GTPase accelerating protein for G(alpha t) in vertebrate photoreceptors. Although the complex is soluble when expressed in vitro, extraction of the endogenous protein from membranes requires detergents. The detergent extracts contain a complex of RGS9-1, G(beta 5), G(alpha t), ... More
Class II histone deacetylases act as signal-responsive repressors of cardiac hypertrophy.
AuthorsZhang CL, McKinsey TA, Chang S, Antos CL, Hill JA, Olson EN,
JournalCell
PubMed ID12202037
The heart responds to stress signals by hypertrophic growth, which is accompanied by activation of the MEF2 transcription factor and reprogramming of cardiac gene expression. We show here that class II histone deacetylases (HDACs), which repress MEF2 activity, are substrates for a stress-responsive kinase specific for conserved serines that regulate ... More
A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples.
Authors Chomczynski P;
JournalBiotechniques
PubMed ID7692896
This report describes a new method for simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. The method is based on the use of a reagent containing phenol and guanidine thiocyanate. A biological sample is homogenized in the reagent and the simultaneous isolation of RNA, DNA and ... More
An IL-4-independent and CD25-mediated function of c-maf in promoting the production of Th2 cytokines.
Authors Hwang Eun Sook; White Ian Alexander; Ho I-Cheng;
JournalProc Natl Acad Sci U S A
PubMed ID12271139
c-maf is a T helper (Th)2 cell-specific transcription factor, which promotes the differentiation of Th2 cells mainly by an IL-4-dependent mechanism. It remains unclear whether c-maf possesses any IL-4-independent function in regulating the production of Th2 cytokines. Here, we provide evidence demonstrating that c-maf, independent of IL-4, is essential for ... More
Light-Regulated Subcellular Translocation of Drosophila TRPL Channels Induces Long-Term Adaptation and Modifies the Light-Induced Current.
Drosophila phototransduction results in the opening of two classes of cation channels, composed of the channel subunits transient receptor potential (TRP), TRP-like (TRPL), and TRPgamma. Here, we report that one of these subunits, TRPL, is translocated back and forth between the signaling membrane and an intracellular compartment by a light-regulated ... More
Requirement of the p130CAS-Crk coupling for metastasis suppressor KAI1/CD82-mediated inhibition of cell migration.
AuthorsZhang XA, He B, Zhou B, Liu L,
JournalJ Biol Chem
PubMed ID12738793
KAI1/CD82 protein is a member of the tetraspanin superfamily and has been rediscovered as a cancer metastasis suppressor. The mechanism of KAI1/CD82-mediated suppression of cancer metastasis remains to be established. In this study, we found that migration of the metastatic prostate cancer cell line Du145 was substantially inhibited when KAI1/CD82 ... More
The nephroblastoma overexpressed gene (NOV/ccn3) protein associates with Notch1 extracellular domain and inhibits myoblast differentiation via Notch signaling pathway.
AuthorsSakamoto K, Yamaguchi S, Ando R, Miyawaki A, Kabasawa Y, Takagi M, Li CL, Perbal B, Katsube K.
JournalJ Biol Chem
PubMed ID12050162
We demonstrate a novel interaction of the nephroblastoma overexpressed gene (NOV), a member of the CCN gene family, with the Notch signaling pathway. NOV associates with the epidermal growth factor-like repeats of Notch1 by the CT (C-terminal cysteine knot) domain. The promoters of HES1 and HES5, which are the downstream ... More
Modifications of cellulose synthase confer resistance to isoxaben and thiazolidinone herbicides in Arabidopsis Ixr1 mutants.
In many higher plants, cellulose synthesis is inhibited by isoxaben and thiazolidinone herbicides such as 5-tert-butyl-carbamoyloxy-3-(3-trifluromethyl) phenyl-4-thiazolidinone. Semidominant mutations at the IXR1 and IXR2 loci of Arabidopsis confer isoxaben and thiazolidinone resistance. Isolation of the IXR1 gene by map-based cloning revealed that it encodes the AtCESA3 isoform of cellulose synthase. ... More
Complementary whole-genome technologies reveal the cellular response to proteasome inhibition by PS-341.
Authors Fleming James A; Lightcap Eric S; Sadis Seth; Thoroddsen Vala; Bulawa Christine E; Blackman Ronald K;
JournalProc Natl Acad Sci U S A
PubMed ID11830665
Although the biochemical targets of most drugs are known, the biological consequences of their actions are typically less well understood. In this study, we have used two whole-genome technologies in Saccharomyces cerevisiae to determine the cellular impact of the proteasome inhibitor PS-341. By combining population genomics, the screening of a ... More
RIM binding proteins (RBPs) couple Rab3-interacting molecules (RIMs) to voltage-gated Ca(2+) channels.
AuthorsHibino H, Pironkova R, Onwumere O, Vologodskaia M, Hudspeth AJ, Lesage F,
JournalNeuron
PubMed ID11988172
Ca(2+) influx through voltage-gated channels initiates the exocytotic fusion of synaptic vesicles to the plasma membrane. Here we show that RIM binding proteins (RBPs), which associate with Ca(2+) channels in hair cells, photoreceptors, and neurons, interact with alpha(1D) (L type) and alpha(1B) (N type) Ca(2+) channel subunits. RBPs contain three ... More
A new class of regulatory genes underlying the cause of pear-shaped tomato fruit.
Authors Liu Jiping; Van Eck Joyce; Cong Bin; Tanksley Steven D;
JournalProc Natl Acad Sci U S A
PubMed ID12242331
A common, recurring theme in domesticated plants is the occurrence of pear-shaped fruit. A major quantitative trait locus (termed ovate) controlling the transition from round to pear-shaped fruit has been cloned from tomato. OVATE is expressed early in flower and fruit development and encodes a previously uncharacterized, hydrophilic protein with ... More
The mouse fidgetin gene defines a new role for AAA family proteins in mammalian development
AuthorsGregory A. Cox*, Connie L. Mahaffey*, Arne Nystuen, Verity A. Letts & Wayne N. Frankel *These authors contributed equally to this work.
JournalNat Genet
PubMed ID11017077
The mouse mutation fidget arose spontaneously in a heteroge-neousalbino stock 1 . This mutant mouse is characterized by aside-to-side head-shaking and circling behaviour, due to reducedor absent semicircular canals 2 . Fidget mice also have small eyes,associated with cell-cycle delay and insufficient growth of theretinal neural epithelium 3,4 , and ... More
Corepressors selectively control the transcriptional activity of PPARgamma in adipocytes.
AuthorsGuan HP, Ishizuka T, Chui PC, Lehrke M, Lazar MA,
JournalGenes Dev
PubMed ID15681609
Peroxisome proliferator-activated receptor gamma (PPARgamma) is the master regulator of adipogenesis as well as the target of thiazolidinedione (TZD) antidiabetic drugs. Many PPARgamma target genes are induced during adipogenesis, but others, such as glycerol kinase (GyK), are expressed at low levels in adipocytes and dramatically up-regulated by TZDs. Here, we ... More
Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155.
AuthorsChung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL,
JournalNucleic Acids Res
PubMed ID16614444
Vector-based RNA interference (RNAi) has emerged as a valuable tool for analysis of gene function. We have developed new RNA polymerase II expression vectors for RNAi, designated SIBR vectors, based upon the non-coding RNA BIC. BIC contains the miR-155 microRNA (miRNA) precursor, and we find that expression of a short ... More
Cytosolic Hydroxymethyldihydropterin Pyrophosphokinase/Dihydropteroate Synthase from Arabidopsis thaliana: A SPECIFIC ROLE IN EARLY DEVELOPMENT AND STRESS RESPONSE.
AuthorsStorozhenko S, Navarrete O, Ravanel S, De Brouwer V, Chaerle P, Zhang GF, Bastien O, Lambert W, Rébeillé F, Van Der Straeten D,
JournalJ Biol Chem
PubMed ID17289662
In plants, 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase/7,8-dihydropteroate synthase (mitHPPK/DHPS) is a bifunctional mitochondrial enzyme, which catalyzes the first two consecutive steps of tetrahydrofolate biosynthesis. Mining the Arabidopsis genome data base has revealed a second gene encoding a protein that lacks a potential transit peptide, suggesting a cytosolic localization of the isoenzyme (cytHPPK/DHPS). When ... More
Involvement of a bifunctional fatty-acyl desaturase in the biosynthesis of the silkmoth, Bombyx mori, sex pheromone.
The straight-chain C(10) to C(18) unsaturated aliphatic compounds containing an oxygenated functional group (aldehyde, alcohol, or acetate ester) derived from saturated C(16) or C(18) fatty acids are a major class of sex pheromone components produced by female moths. In the biosynthesis of these pheromone components, various combinations of limited chain-shortening ... More
Endothelial induction of fgl2 contributes to thrombosis during acute vascular xenograft rejection.
AuthorsGhanekar A, Mendicino M, Liu H, He W, Liu M, Zhong R, Phillips MJ, Levy GA, Grant DR,
JournalJ Immunol
PubMed ID15100314
Thrombosis is a prominent feature of acute vascular rejection (AVR), the current barrier to survival of pig-to-primate xenografts. Fibrinogen-like protein 2 (fgl2/fibroleukin) is an inducible prothrombinase that plays an important role in the pathogenesis of fibrin deposition during viral hepatitis and cytokine-induced fetal loss. We hypothesized that induction of fgl2 ... More
Analysis of gene expression in ceca of Helicobacter hepaticus-infected A/JCr mice before and after development of typhlitis.
AuthorsMyles MH, Livingston RS, Livingston BA, Criley JM, Franklin CL,
JournalInfect Immun
PubMed ID12819073
The inflammatory bowel diseases, Crohn's disease and ulcerative colitis, are chronic inflammatory disorders of the gastrointestinal tract. The causes of these diseases remain unknown; however, prevailing theories suggest that chronic intestinal inflammation results from a dysregulated immune response to ubiquitous bacterial antigens. While a substantial body of data has been ... More
The association between aneurysm formation and systemic hypertension in Takayasu's arteritis.
AuthorsSharma S, Rajani M, Kamalakar T, Kumar A, Talwar KK,
JournalClin Radiol
PubMed ID1976468
We have studied the incidence and patterns of aneurysm formation in 88 consecutive patients with Takayasu's arteritis by utilizing digital subtraction pan-aortoarteriography by the intravenous and/or intra-arterial route. Seventy-two patients underwent initial intravenous angiography of which 64 (89%) were diagnostic. All the intra-arterial studies (n = 24) were diagnostically adequate. ... More
A universal assay for detection of oncogenic fusion transcripts by oligo microarray analysis.
AuthorsSkotheim RI, Thomassen GO, Eken M, Lind GE, Micci F, Ribeiro FR, Cerveira N, Teixeira MR, Heim S, Rognes T, Lothe RA,
JournalMol Cancer
PubMed ID19152679
BACKGROUND: The ability to detect neoplasia-specific fusion genes is important not only in cancer research, but also increasingly in clinical settings to ensure that correct diagnosis is made and the optimal treatment is chosen. However, the available methodologies to detect such fusions all have their distinct short-comings. RESULTS: We describe ... More
Erythroid expression of the human alpha-spectrin gene promoter is mediated by GATA-1- and NF-E2-binding proteins.
AuthorsBoulanger L, Sabatino DE, Wong EY, Cline AP, Garrett LJ, Garbarz M, Dhermy D, Bodine DM, Gallagher PG,
JournalJ Biol Chem
PubMed ID12196550
alpha-Spectrin is a highly expressed membrane protein critical for the flexibility and stability of the erythrocyte. Qualitative and quantitative defects of alpha-spectrin are present in the erythrocytes of many patients with abnormalities of red blood cell shape including hereditary spherocytosis and elliptocytosis. We wished to determine the regulatory elements that ... More
Roles of neurotransmitter in synapse formation: development of neuromuscular junctions lacking choline acetyltransferase.
Activity-dependent and -independent signals collaborate to regulate synaptogenesis, but their relative contributions are unclear. Here, we describe the formation of neuromuscular synapses at which neurotransmission is completely and specifically blocked by mutation of the neurotransmitter-synthesizing enzyme choline acetyltransferase. Nerve terminals differentiate extensively in the absence of neurotransmitter, but neurotransmission plays ... More
Genetic analysis of adipogenesis through peroxisome proliferator-activated receptor gamma isoforms.
AuthorsMueller E, Drori S, Aiyer A, Yie J, Sarraf P, Chen H, Hauser S, Rosen ED, Ge K, Roeder RG, Spiegelman BM,
JournalJ Biol Chem
PubMed ID12200443
Peroxisome proliferator-activated receptor (PPAR) gamma is a nuclear receptor that is a key regulator of adipogenesis and is present in two isoforms generated by alternative splicing, PPARgamma1 and PPARgamma2. Studies of the ability of each isoform to stimulate fat differentiation have yielded ambiguous results, in part because PPARgamma stimulates its ... More
The fibroblast growth factor receptor-4 Arg388 allele is associated with prostate cancer initiation and progression.
AuthorsWang J, Stockton DW, Ittmann M,
JournalClin Cancer Res
PubMed ID15448004
PURPOSE: Increased expression of fibroblast growth factors that can activate the fibroblast growth factor receptor-4 (FGFR-4) occurs in a substantial fraction of human prostate cancers in vivo. A germline polymorphism of the FGFR-4 gene resulting in expression of arginine at codon 388 (Arg388) is associated with aggressive disease in patients ... More
A role for the granzyme B inhibitor serine protease inhibitor 6 in CD8+ memory cell homeostasis.
AuthorsPhillips T, Opferman JT, Shah R, Liu N, Froelich CJ, Ashton-Rickardt PG,
JournalJ Immunol
PubMed ID15356127
Generation and maintenance of protective immunological memory is the goal of vaccination programs. It has recently become clear that CD8+ memory T cells are derived directly from CTLs. The mechanisms underlying this transformation and the subsequent survival of memory cells are not completely understood. However, some effector molecules required by ... More
NF-kappa B-inducing kinase is a common mediator of IL-17-, TNF-alpha-, and IL-1 beta-induced chemokine promoter activation in intestinal epithelial cells.
AuthorsAwane M, Andres PG, Li DJ, Reinecker HC
JournalJ Immunol
PubMed ID10228009
IL-17 expression is restricted to activated T cells, whereas the IL-17R is expressed in a variety of cell types including intestinal epithelial cells. However, the functional responses of intestinal epithelial cells to stimulation with IL-17 are unknown. Moreover, the signal transduction pathways activated by the IL-17R have not been characterized. ... More
A new member of the mouse prolactin (PRL)-like protein-C subfamily, PRL- like protein-C alpha: structure and expression.
AuthorsDai G, Chapman BM, Liu B, Orwig KE, Wang D, White RA, Preuett B, Soares MJ
JournalEndocrinology
PubMed ID9832456
In this study, we establish the presence of a unique member of the PRL- like protein-C (PLP-C) subfamily in the mouse, PLP-C alpha, characterize its complementary DNA and gene, and map its chromosomal location and pattern of expression during pregnancy. Mouse PLP-C alpha encodes for a 239 amino acid protein ... More
The effects of glucose concentration on the reciprocal regulation of rRNA promoters in Plasmodium falciparum.
AuthorsFang J, Sullivan M, McCutchan TF,
JournalJ Biol Chem
PubMed ID14570919
The developmental progression of Plasmodium falciparum is remarkably sensitive to glucose concentration. We have investigated the effects of glucose concentration on the parasite development cycle as reflected by changes of ribosomal RNA (rRNA) transcription. We showed that glucose starvation differentially affects transcriptional control of the rRNA genes by sharply repressing ... More
Cloning and characterization of a GABAA receptor gamma 2 subunit variant.
AuthorsJin P, Zhang J, Rowe-Teeter C, Yang J, Stuve LL, Fu GK,
JournalJ Biol Chem
PubMed ID14593118
We have cloned a novel GABAA receptor gamma2 subunit variant, named gamma2XL. gamma2XL contains an alternatively spliced exon, resulting in the addition of 40 amino acids to the N-terminal extra-cellular domain between S171 and Y172. We show that gamma2XL fails to localize to the cell surface when it is co-expressed ... More
Use of Sindbis virus-mediated RNA interference to demonstrate a conserved role of Broad-Complex in insect metamorphosis.
AuthorsUhlirova M, Foy BD, Beaty BJ, Olson KE, Riddiford LM, Jindra M,
JournalProc Natl Acad Sci U S A
PubMed ID14668449
The transcription factor Broad-Complex (BR-C) is required for differentiation of adult structures as well as for the programmed death of obsolete larval organs during metamorphosis of the fruit fly Drosophila melanogaster. Whether BR-C has a similar role in other holometabolous insects could not be proven without a loss-of-function genetic test, ... More
Phosphorylation of rat spermatidal protein TP2 by sperm-specific protein kinase A and modulation of its transport into the haploid nucleus.
AuthorsUllas KS, Rao MR,
JournalJ Biol Chem
PubMed ID14514679
Transition protein 2 (TP2), which is expressed during stages 12-15 of mammalian spermiogenesis, has been shown to undergo phosphorylation immediately after its synthesis. We reported earlier that TP2 is phosphorylated in vitro at threonine 101 and serine 109 by the salt extract of sonication-resistant (elongating and elongated) spermatid nuclei and ... More
The neuropeptide galanin augments lobuloalveolar development.
Mammary lobuloalveolar development during pregnancy is controlled by ovarian sex steroids and pituitary prolactin release. In organ culture these hormones are incapable of reproducing the density and size of lobuloalveoli seen in mice, suggesting the existence of other undiscovered factors. We showed previously that galanin knockout mice fail to lactate ... More
[Consultation, pedagogy and therapy]
AuthorsLempp R,
JournalPadiatr Padol
PubMed ID1250620
In child psychiatry the discrepancey between the diagnostic and therapeutic possibilities can often be disappointing, and thus, especially the young people involved demand education in approved psychotherapeutic techniques. This seems to be more important than education in common diagnosis and consultation, but the term psychotherapy is much too restricted and ... More
Differential expression of anti-angiogenic factors and guidance genes in the developing macula.
AuthorsKozulin P, Natoli R, O'Brien KM, Madigan MC, Provis JM,
JournalMol Vis
PubMed ID19145251
PURPOSE: The primate retina contains a specialized, cone-rich macula, which mediates high acuity and color vision. The spatial resolution provided by the neural retina at the macula is optimized by stereotyped retinal blood vessel and ganglion cell axon patterning, which radiate away from the macula and reduce shadowing of macular ... More
NADPH oxidase contributes to coronary endothelial dysfunction in the failing heart.
AuthorsZhang P, Hou M, Li Y, Xu X, Barsoum M, Chen Y, Bache RJ,
JournalAm J Physiol Heart Circ Physiol
PubMed ID19168727
Increased reactive oxygen species (ROS) produced by the failing heart can react with nitric oxide (NO), thereby decreasing NO bioavailability. This study tested the hypothesis that increased ROS generation contributes to coronary endothelial dysfunction in the failing heart. Congestive heart failure (CHF) was produced in six dogs by ventricular pacing ... More
MicroRNA expression profiling using microarrays.
AuthorsLiu CG, Calin GA, Volinia S, Croce CM,
JournalNat Protoc
PubMed ID18388938
Microarray technology is a powerful high-throughput tool capable of monitoring the expression of thousands of small noncoding RNAs at once within tens of samples processed in parallel in a single experiment. To conduct a genome-wide analysis of miRNA expression of normal and disease samples, such as cancer, and to distinguish ... More
P21 functions to maintain quiescence of p27-deficient hepatocytes.
AuthorsKwon YH, Jovanovic A, Serfas MS, Kiyokawa H, Tyner AL,
JournalJ Biol Chem
PubMed ID12202477
Hepatocytes rarely proliferate in the healthy adult liver. We explored the roles of the cyclin kinase inhibitors p21 and p27 in maintaining hepatocyte quiescence. p27 is expressed throughout the wild-type liver, but the related protein p21 was not detected. However, p21 was detected in livers of p27-deficient mice. Increased p21 ... More
Generation of soluble leptin receptor by ectodomain shedding of membrane-spanning receptors in vitro and in vivo.
AuthorsGe H, Huang L, Pourbahrami T, Li C,
JournalJ Biol Chem
PubMed ID12270921
Leptin is an adipocyte-derived hormone with potent effects on food intake and body weight. Genetically obese rodents with mutations of leptin or leptin receptor develop morbid obesity and diabetes. The receptor for leptin, OB-R, is alternatively spliced to at least five transcripts, encoding receptors designated OB-Ra, -b, -c, -d, and ... More
Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs.
AuthorsRandall G, Grakoui A, Rice CM,
JournalProc Natl Acad Sci U S A
PubMed ID12518066
RNA interference is a cellular process of gene silencing in which small duplexes of RNA specifically target a homologous sequence for cleavage by cellular ribonucleases. The introduction of approximately 22-nt small interfering RNAs (siRNAs) into mammalian cells can specifically silence cellular mRNAs without induction of the nonspecific IFN responses that ... More
Suppressor of Cytokine Signaling 3 Regulates Proliferation and Activation of T-helper Cells.
Suppressors of cytokine signaling (SOCS) have been implicated in regulation of T-cell activation and cytokine-mediated differentiation of T-helper cells. In this study we have characterized the pattern of SOCS expression in naïve and activated primary T-helper cells, examined whether expression of SOCS genes is regulated by cytokine or T-cell receptor ... More
Rescuing qkV dysmyelination by a single isoform of the selective RNA-binding protein QKI.
AuthorsZhao L, Tian D, Xia M, Macklin WB, Feng Y,
JournalJ Neurosci
PubMed ID17079655
Alternative splicing of the qkI transcript generates multiple isoforms of the selective RNA-binding protein QKI, which play key roles in controlling the homeostasis of their mRNA targets. QKI deficiency in oligodendrocytes of homozygous quakingviable (qkV/qkV) mutant mice results in severe hypomyelination, indicating the essential function of QKI in myelinogenesis. However, ... More