HEPES (1 M)

Zitierungen und Referenzen (5)

Gibco™

HEPES (1 M)

Name: HEPES (1 M)
SKU: 15630106
Price: 58.75 EUR

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Katalognummer	Menge
15630106	20 mL
15630080	100 mL

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Katalognummer 15630106

Preis (EUR)

58,75

Each

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Menge:

20 mL

Preis (EUR)

58,75

Each

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Gibco™ HEPES N-2-Hydroxyethylpiperazin-N-2-ethansulfonsäure) ist ein zwitterionisches, organisches, chemisches Puffermittel, das häufig in Zellkulturmedien eingesetzt wird. Die Zugabe von 10 bis 25 mM HEPES-Puffer sorgt für zusätzliche Pufferkapazität, wenn sich die Zellkultur für längere Manipulationsperioden außerhalb des CO₂-Inkubators befindet. HEPES-Puffer ist eine gute Wahl für viele Zellkultursysteme, da es membranundurchlässig ist, weniger Auswirkungen auf biochemische Reaktionen hat, chemisch und enzymatisch stabil ist und sich durch geringe Licht- und UV-Absorption auszeichnet.

cGMP-Fertigung an zwei Standorten
Gibco™ HEPES wird in einer cGMP-konformen Einrichtung hergestellt, die sich sich in Paisley, Schottland (Großbritannien) befindet. Die Einrichtung ist von der US-Arzneimittelbehörde FDA als Hersteller von Medizinprodukten zugelassen und nach ISO 13485-zertifiziert. Um lückenlose Versorgung zu sichern, bieten wir ein identisches Gibco™ HEPES-Produkt an, das an unserem Standort in Grand Island (USA) hergestellt wird (15630-080). Diese Einrichtung ist von der US-Arzneimittelbehörde FDA als Hersteller von Medizinprodukten zugelassen und nach ISO 13485-zertifiziert.

Für die Verwendung in der Forschung oder die Weiterverarbeitung. Nicht zur Diagnosestellung oder direkten Anwendung bei Menschen oder Tieren geeignet.

Specifications

Chemischer Name oder MaterialZwitterionic Organic Chemical Buffer

ZusammensetzungN-2-Hydroxyethylpiperazine-N-2-Ethane Sulfonic Acid

Empfohlene LagerungStorage conditions: 2 – 8°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture

SterilitätSteril gefiltert

SterilisationsmethodeSterile-filtered

Zur Verwendung mit (Anwendung)Chromatin-Biologie, Säugetierzellkultur

GüteBiochemie

Physikalische FormFlüssigkeit

Menge20 mL

LösungstypBuffer

Unit SizeEach

Häufig gestellte Fragen (FAQ)

Based on the Sodium bicarbonate levels in the medium, what CO2 % is recommended?

If the media formulation contains:

NaHCO3 (g/L) < 1.5, it needs CO2 at 4%;
NaHCO3 (g/L) 1.5 – 2.2, it needs CO2 at 5%;
NaHCO3 (g/L) 2.2 - 3.4, it needs CO2 at 7%;
NaHCO3 (g/L) > 3.5, it needs CO2 at 10% .

*There are some exceptions. Gibco DMEM has always been made according to Dulbecco’s original published formulation, with 3.7 g/L sodium bicarbonate. Customers have been using this medium in CO2 incubators ranging from 5-10% CO2 for decades, usually with no trouble maintaining physiological pH. This also depends on the cell type. Once cells are growing, the pH will drop (due to metabolic accumulation of lactic acid).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do high or low levels of sodium bicarbonate affect the pH of my tissue culture media?

Sodium bicarbonate is necessary to control the pH of the solution. To maintain physiological pH, the concentration of the sodium bicarbonate in the medium must be matched with the right level of CO2 in the atmosphere above the medium in the incubator*.

If the sodium bicarbonate is high and the CO2 concentration is low, the pH will become alkaline. This is something that you will see in a bottle of media when it is exposed to air for long periods or when there is a lot of head space in the bottle. The media color will be pinkish or purplish. When this media is put back into a CO2 incubator, the color/pH will change back to the normal orangeish/reddish color or physiological pH.

If the sodium bicarbonate is low and the CO2 is high, the pH will become acidic. This is something you will see when you put medium into a 5% or 10% CO2 incubator.

*There are some exceptions. For instance, Gibco DMEM has always been made according to Dulbecco’s original published formulation, with 3.7g/L sodium bicarbonate. Customers have been using this medium in CO2 incubators ranging from 5-10% CO2 for decades, usually with no trouble maintaining physiological pH. this also depends on the cell type, Once cells are growing, the pH will drop (due to metabolic accumulation of lactic acid).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

My Gibco HEPES (1M) (Cat. No. 15630080) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is HEPES (1 M) in distilled water or in saline solution?

1M HEPES is in water with a pH range of 7.2 - 7.5.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the endotoxin specification for HEPES (1 M) (Cat. Nos. 15630080, 15630106, and 15630130)?

Sorry, we do not test endotoxin levels for this product.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Zitierungen und Referenzen (5)

Zitierungen und Referenzen

Abstract

A solution for highly efficient electroporation of primary cytotoxic T lymphocytes.

Authors:Alawar N,Schirra C,Hohmann M,Becherer U

Journal:BMC biotechnology

PubMed ID:38532411

BACKGROUND: Cytotoxic T lymphocytes (CTLs) are central players in the adaptive immune response. Their functional characterization and clinical research depend on efficient and reliable transfection. Although various methods have been utilized, electroporation remains the preferred technique for transient gene over-expression. However, the efficiency of electroporation is reduced for human and ... More

Thiazolidinedione activation of peroxisome proliferator-activated receptor gamma can enhance mitochondrial potential and promote cell survival.

Authors: Wang Y Lynn; Frauwirth Kenneth A; Rangwala Shamina M; Lazar Mitchell A; Thompson Craig B;

Journal:J Biol Chem

PubMed ID:12082115

'Thiazolidinediones (TZDs) are widely used for treatment of type 2 diabetes mellitus. Peroxisome proliferator-activated receptor gamma (PPAR gamma) is the molecular target of TZDs and is believed to mediate the apoptotic effects of this class of drugs in a variety of cell types, including B and T lymphocytes. The finding ... More

Role of hepatic transporters in the disposition and hepatotoxicity of a HER2 tyrosine kinase inhibitor CP-724,714.

Authors:Feng B, Xu JJ, Bi YA, Mireles R, Davidson R, Duignan DB, Campbell S, Kostrubsky VE, Dunn MC, Smith AR, Wang HF,

Journal:Toxicol Sci

PubMed ID:19223659

'CP-724,714, a potent and selective orally active HER2 tyrosine kinase inhibitor, was discontinued from clinical development due to unexpected hepatotoxicity in cancer patients. Based on the clinical manifestation of the toxicity, CP-724,714 likely exerted its hepatotoxicity via both hepatocellular injury and hepatobiliary cholestatic mechanisms. The direct cytotoxic effect, hepatobiliary disposition ... More

Molecular rearrangements of the extracellular vestibule in NMDAR channels during gating.

Authors: Sobolevsky Alexander I; Beck Christine; Wollmuth Lonnie P;

Journal:Neuron

PubMed ID:11779481

Many N-methyl-D-aspartate receptor (NMDAR) channel blockers that have therapeutic potential can be trapped in the closed state. Using a combination of the substituted cysteine accessibility method and open channel blockers, we found that the M3 segment forms the core of the extracellular vestibule, including a deep site for trapping blockers. ... More

Activation of human monoamine oxidase B gene expression by a protein kinase C MAPK signal transduction pathway involves c-Jun and Egr-1.

Authors: Wong Wai K; Ou Xiao-Ming; Chen Kevin; Shih Jean C;

Journal:J Biol Chem

PubMed ID:11956220

Monoamine oxidases (MAO) A and B deaminate a number of biogenic amines. Aberrant expression of MAO is implicated in several psychiatric and neurogenerative disorders. In this study, we have shown that phorbol 12-myristate 13-acetate (PMA) increases human MAO B, but not MAO A, gene expression. The sequence between -246 and ... More