Gentamicina (10 mg/ml)
Gentamicina (10 mg/ml)
Citas y referencias (4)
Gibco™

Gentamicina (10 mg/ml)

El sulfato de gentamicina es un antibiótico soluble en agua purificado originalmente a partir del hongo Micromonospora purpurea. La gentamicinaMás información
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Número de catálogoCantidad
1571007210 x 10 mL
1571006410 mL
Número de catálogo 15710072
Precio (USD)
252,45
Each
Añadir al carro de la compra
Cantidad:
10 x 10 mL
Precio (USD)
252,45
Each
Añadir al carro de la compra
El sulfato de gentamicina es un antibiótico soluble en agua purificado originalmente a partir del hongo Micromonospora purpurea. La gentamicina actúa uniéndose a la subunidad 30S del ribosoma bacteriano, lo que conduce a la inhibición de la síntesis de proteínas y a la muerte de las bacterias susceptibles. La gentamicina Gibco™ es eficaz contra una amplia variedad de bacterias grampositivas y gramnegativas, y se utiliza para la prevención de la contaminación bacteriana de cultivos celulares. La concentración de trabajo recomendada varía entre 0,5 y 50 μg/ml. Ofrecemos una amplia variedad de antibióticos y antimicóticos para aplicaciones de cultivo celular.

Uso del producto
Para uso exclusivo en investigación: no diseñado para uso diagnóstico o terapéutico en animales o humanos.

Producción según las prácticas correctas de fabricación actuales en dos instalaciones
La gentamicina Gibco™ se fabrica en una instalación que cumple con las buenas prácticas de fabricación actuales, ubicada en Grand Island, Nueva York (EE. UU.). Las instalaciones están registradas en la FDA como fabricante de dispositivos médicos y están certificadas según la norma ISO 13485. Para mantener la continuidad de la cadena de suministro, podemos ofrecer un producto de gentamicina Gibco™ comparable que fabricamos en nuestras instalaciones de Escocia (15710-049). Estas instalaciones están registradas en la FDA como fabricante de dispositivos médicos y están certificadas según la norma ISO 13485.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones
Concentración10 mg/mL
Tipo de cultivoMammalian Cell Culture
Para utilizar con (aplicación)Prevención de la contaminación del cultivo celular
Cantidad10 x 10 mL
Duración de almacenamiento24 Months
Condiciones de envíoTemperatura ambiente
FormularioLíquido
Tipo de productoAntibiotic
EsterilidadEstéril con filtro
Unit SizeEach
Contenido y almacenamiento
Condiciones de almacenamiento: De 15 a 30 °C
Condiciones de envío: Ambiente
Vida útil: 24 meses a partir de la fecha de fabricación

Preguntas frecuentes

If Gentamicin (10 mg/mL) is accidentally stored at 2-8 degrees C, would it affect the stability of the antibiotic?

No, storing Gentamicin solution for several days at 2-8 degrees C will not have any negative impact on its performance or stability. However, as Gentamicin solution has been shown to be stable at room temperature, the recommended storage temperature is ~25 degrees C.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I decontaminate my cultures?

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What antibiotics do you offer to help control or eliminate cell culture contamination?

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Citations & References (4)

Citations & References
Abstract
Comparing Effects of Transforming Growth Factor ß1 on Microglia From Rat and Mouse: Transcriptional Profiles and Potassium Channels.
Authors:Lively S, Lam D, Wong R, Schlichter LC
Journal:Front Cell Neurosci
PubMed ID:29780305
'The cytokine, transforming growth factor ß1 (TGFß1), is up-regulated after central nervous system (CNS) injuries or diseases involving microglial activation, and it has been proposed as a therapeutic agent for treating neuroinflammation. Microglia can produce and respond to TGFß1. While rats and mice are commonly used for studying neuroinflammation, very ... More
Re-Defining Stem Cell-Cardiomyocyte Interactions: Focusing on the Paracrine Effector Approach.
Authors:Mahapatra S, Martin D, Gallicano GI
Journal:J Stem Cells Regen Med
PubMed ID:30018469
'Stem cell research for treating or curing ischemic heart disease has, till date, culminated in three basic approaches: the use of induced pluripotent stem cell (iPSC) technology; reprogramming cardiac fibroblasts; and cardiovascular progenitor cell regeneration. As each approach has been shown to have its advantages and disadvantages, exploiting the advantages ... More
Microglia Responses to Pro-inflammatory Stimuli (LPS, IFN?+TNFa) and Reprogramming by Resolving Cytokines (IL-4, IL-10).
Authors:Lively S, Schlichter LC
Journal:Front Cell Neurosci
PubMed ID:30087595
Microglia respond to CNS injuries and diseases with complex reactions, often called "activation." A pro-inflammatory phenotype (also called classical or M1 activation) lies at one extreme of the reactivity spectrum. There were several motivations for this study. First, bacterial endotoxin (lipopolysaccharide, LPS) is the most commonly used pro-inflammatory stimulus for ... More
Polarized Human Retinal Pigment Epithelium Exhibits Distinct Surface Proteome on Apical and Basal Plasma Membranes.
Authors:Khristov V, Wan Q, Sharma R, Lotfi M, Maminishkis A, Bharti K
Journal:Methods Mol Biol
PubMed ID:29264809
Surface proteins localized on the apical and basal plasma membranes are required for a cell to sense its environment and relay changes in ionic, cytokine, chemokine, and hormone levels to the inside of the cell. In a polarized cell, surface proteins are differentially localized on the apical or the basolateral ... More