Recombinant Protein G Agarose

Catalog number: 15920010

Novex™  Related applications: Antibody Purification | Protein Assays and Analysis | Protein Purification & Isolation

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Description

Recombinant Protein G (rProtein G) Agarose is a ready-to-use affinity chromatography matrix supplied as a 50% slurry (in phosphate buffered saline (PBS), pH 7.0; 20% ethanol). It is used for the purification of IgG from most mammalian species (see table), and does not bind IgM, IgA, IgE, or serum albumin. The rProtein G is a recombinant form of streptococcal protein G engineered to eliminate nonspecific binding to serum albumin, and is immobilized on 4% agarose beads. rProtein G Agarose has a binding capacity of approximately 20 mg of human IgG per mL of beads. rProtein G Agarose binds to a broader range of IgG isotypes than react with protein A. The binding characteristics and stability of rProtein G Agarose facilitate rapid and repeated use for affinity chromatography.

Using rProtein G Agarose
To purify IgG, load crude samples (serum, ascites fluid, or hybridoma supernatant) onto a column of rProtein G Agarose under physiologic conditions of salt and pH. Non-IgG components do not bind to the beads and elute in the flow through fraction. Purified IgG is then eluted with a low pH buffer.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Suspension
Column Type: Affinity Column
Product Size: 5 mL
Immobilized Phase: Protein G
Shipping Condition: Wet Ice

Contents & storage

rProtein G Agarose is supplied as a 50% suspension in PBS (pH 7.0), 20% ethanol. Store at +4°C.

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Manuals & protocols

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