TRIzol® Max™ Bacterial RNA Isolation Kit

Catalog number: 16096040

Ambion™  Related applications: RNA Extraction

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Description

The TRIzol® Max™ Bacterial RNA Isolation Kit provides a simple and reliable method to improve the isolation of intact total RNA from gram-positive and gram-negative bacteria. The kit utilizes both the Max™ Bacterial Enhancement Reagent and TRIzol® Reagent to inactivate endogenous RNases and promote protein denaturing, improving RNA quality and integrity. Isolation of RNA takes about an hour.

Key Features of the TRIzol® Max™ Bacterial RNA Isolation Kit:

• Formulated for the isolation of RNA from both Gram-positive and Gram-negative bacteria
• Uses two technologies for maximum RNA yield and purity
• Minimizes time required for RNA isolation by eliminating enzymatic and mechanical lytic steps

Two Products in One Kit
The TRIzol® Max™ Bacterial RNA Isolation Kit combines Max™ Bacterial Enhancement Reagent with TRIzol® Reagent. A 5-minute pre-treatment of bacteria with Max™ Bacterial Enhancement Reagent, containing chelating agents, detergent and buffer, denatures bacterial proteins and effectively deactivates endogenous RNases. Subsequent bacterial lysis by TRIzol® Reagent generates a high-quality, minimally degraded RNA product.

Purified RNA is Ideal for Use with a Variety of Applications and Products
Bacterial RNA isolated using the TRIzol® Max™ Bacterial RNA Isolation Kit is suitable for downstream applications such as real-time PCR (qPCR), RT-PCR, northern blotting, nuclease protection assays, cDNA synthesis, microarray analysis, and dot blot hybridization.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Sample Type (General): Bacteria
High Throughput Compatibility: Not High Throughput-Compatible (Manual)
Downstream Application: Reverse Transcriptase PCR (RT-PCR), Cloning, Real-Time Quantitative PCR (qPCR), Nuclease Protection Assays, cDNA Library Construction, Northern Blotting
Scale: Scaleable
System: TRIzol
Quantity: 1
Product Size: 200 preps
Final Product: Total RNA, micro RNA, Transcriptome RNA
Yield (up to): 70 µg
Purification Time: 1 hr
Number of Reactions: 200 Preps
Isolation Technology: Organic Extraction
Sample Type (Specific): Bacteria (Gram-Positive), Bacteria (Gram-Negative)
Starting Material (Amount): Up to 1 x 10^8 cells

Contents & storage

Includes 200mL TRIzol® Reagent and 2 x 20 mL Max Bacterial Enhancement Reagent.

Store TRIzol® Reagent at 2°C to 25°C. Max Bacterial Enhancement Reagent may be stored at room temperature.

Documents

Manuals & protocols

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