Pierce™ Gaussia Luciferase Flash Assay Kit

Catalog number: 16158

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Pierce Gaussia Luciferase Flash Assay Kit provides researchers with the most sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

Features of the Gaussia Luciferase Flash Assay Kit:

Sensitive—1000 times greater sensitivity allows utilization of smaller numbers of cells
Cost effective—highest sensitivity assays result in decreased reagent consumption
Secreted luciferase—allows real-time assays and kinetic studies without destroying cells
Time-saving—assays using secreted luciferases require minimal sample handling
Automation-friendly—amenable to high throughput screens
Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
Safe—allows one to perform non-radioactive assays

This Flash Assay Kit contains reagents for measuring the activity of Gaussia luciferase in mammalian cell culture media and lysates. When used with Thermo Scientific Gaussia Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity. Gaussia luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced by Gaussia is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.

Includes:
Cell lysis buffer, reaction buffer and substrate

Requires:
Gaussia luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.

Applications:
• Promoter studies for analyzing cis- regulatory elements and trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

Gaussia luciferase is a 20kDa protein from the marine copepod, Gaussia princeps. The bioluminescent enzyme is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output generated by the luciferase reaction can be correlated to the amount of Gaussia luciferase protein produced and used to determine the activity of the promoter driving Gaussia expression. The signal produced by Gaussia luciferase shows strong flash kinetics and is considerably greater than flash signals from either firefly or Renilla luciferases assayed under similar conditions. The assay kit is optimized for use with our Gaussia Luc Vectors but is fully compatible with other Gaussia luciferases and derivatives that use coelenterazine as a substrate. (For glow assays, use our Gaussia-Dura Luc Vectors.)

More Product Data
Highly sensitive multiplex luciferase reporter assays
Luciferase assays in hard-to-transfect Jurkat cells
Monitoring neuronal differentiation using multiplexed luciferase reporters
Activation of the antioxidant response pathway by pesticide chemicals
Versatile luciferases: microplate luminometers and flash luciferase assays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 96-well plate, 384-well plate
Substrate: Coelenterazine
Technique: Enhanced Chemiluminescence
Assay Type: Reporter Enzyme, Luciferase Reporter Assay
Product Size: 100 reactions
Target Enzyme: Luciferase, Gaussia Luciferase
Substrate Type: Luciferase Substrate
Compatible Cells: Mammalian Cells
Detection Method: Bioluminescent
Substrate Properties: Chemical Substrate
For Use With (Equipment): Luminometer (Microplate)

Contents & storage

Sufficient For: 100 luciferase assays in microplate wells
• Gaussia Flash Assay Buffer, 5 mL (store at 4°C)
• 100X Coelenterazine, 50 µL (store at -80°C)
• Luciferase Cell Lysis Buffer (2X), 6 mL (store at room temperature)

Kit may be stored at -80°C, or individually at indicated temperatures.

Documents

Manuals & protocols

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