Pierce™ Cypridina Luciferase Flash Assay Kit

Catalog number: 16168

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Pierce Cypridina Luciferase Flash Assay Kit provides researchers with a highly sensitive assay for transcriptional activity of regulatory elements in mammalian cell culture media and whole cell lysate.

Features of the Cypridina Luciferase Flash Assay Kit:

Sensitive—high sensitivity allows utilization of smaller numbers of cells
Cost effective—sensitive assays result in decreased reagent consumption
Secreted luciferase—allows real-time assays and kinetic studies without destroying cells
Ideal for co-transfection—uses a different substrate than Renilla, Gaussia, or firefly luciferases
Compatible—assay reagents compatible with other Cypridina or Vargula luciferases
Time-saving—assays using secreted luciferases require minimal sample handling
Automation-friendly—amenable to high throughput screens
Convenient—contains a universal cell lysis buffer and optimized flash assay reagent
Safe—allows one to perform non-radioactive assays

This Flash Assay Kit contains reagents for measuring the activity of Cypridina luciferase in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of promoter or pathway activity. Cypridina luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. The signal produced by Cypridina is considerably greater than signal from either firefly or Renilla luciferases assayed under similar conditions.

Includes:
Cell lysis buffer, reaction buffer and substrate

Requires:
Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.

Applications:
• Promoter studies for analyzing cis- regulatory elements and trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

The Cypridina luciferase is a 61kDa protein from the marine ostracod, Cypridina noctiluca. Cypridina luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output captured using a luminometer can be correlated with the amount of Cypridina luciferase protein produced and used to determine the activity of the promoter driving Cypridina expression. The Cypridina gene in Thermo Scientific Cypridina Luc Vectors is modified for expression in mammalian cells.

The assay kit is optimized for use with our Cypridina Luc Vectors but is fully compatible with any luciferases that use Vargulin as a substrate. Because Cypridina uses a different substrate than Renilla, Gaussia, or firefly luciferases, co-transfection and assays with up to three reporters are possible. The signal produced by Cypridina shows strong flash kinetics and is considerably greater than flash signals from either firefly or Renilla luciferases assayed under similar conditions.

More Product Data
Highly sensitive multiplex luciferase reporter assays
Luciferase assays in hard-to-transfect Jurkat cells
Monitoring neuronal differentiation using multiplexed luciferase reporters
Activation of the antioxidant response pathway by pesticide chemicals
Versatile luciferases: microplate luminometers and flash luciferase assays
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 96-well plate, 384-well plate
Substrate: Vargulin (Cypridina Luciferin)
Technique: Enhanced Chemiluminescence
Assay Type: Reporter Enzyme, Luciferase Reporter Assay
Product Size: 100 reactions
Target Enzyme: Luciferase, Cypridina Luciferase
Substrate Type: Luciferase Substrate
Compatible Cells: Mammalian Cells
Detection Method: Bioluminescent
Substrate Properties: Chemical Substrate
For Use With (Equipment): Luminometer (Microplate)

Contents & storage

• Cypridina Flash Assay Buffer, 5 mL, store at 4°C
• Vargulin (1X), 5 µL, store at -20°C
• 2X Cell Lysis Buffer, 6 mL, store at room temperature

Upon receipt store kit at -20°C or store individual components as indicated above.

Documents

Manuals & protocols