Pierce™ Cypridina Luciferase Glow Assay Kit

Catalog number: 16170

Thermo Scientific™  Related applications: Protein Assays and Analysis

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Description

The Thermo Scientific Pierce Cypridina Luciferase Glow Assay Kit provides an extremely bright and stable bioluminescence signal (half-life greater than 1 hour) in the presence of Cypridina luciferase reporters.

Features of the Cypridina Luciferase Glow Assay Kit:

Sensitive—highly sensitive detection of Cypridina luciferase activity
Secreted luciferase—allows real-time assays and kinetic studies without destroying cells
Stable—reagents provide increased signal stability compared to flash assays
Simple—does not require a luminometer with injectors
Compatible—assay reagents compatible with other Cypridina or Vargula luciferases
Automation-friendly—amenable to high throughput assays
Convenient—contains a universal cell lysis buffer and optimized glow assay reagent
Safe—allows one to perform non-radioactive assays

This Pierce Luciferase Glow Assay Kit contains reagents for measuring Cypridina luciferase activity in mammalian cell culture media and lysates. When used with Thermo Scientific Cypridina Luc Vectors, the kit provides an extremely sensitive bioluminescent reporter assay system for secreted or intracellular detection of luciferase activity. The light output generated by the luciferase reaction can be correlated to the amount of luciferase protein produced, which in turn is proportional to the activity of the promoter driving the luciferase expression. The kit can be used to detect the activity of any Cypridina luciferases or derivatives that use Vargulin as a substrate.

Includes:
Cell lysis buffer, reaction buffer and substrate

Requires:
Cypridina luciferase and luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers.

Applications:
• Promoter studies for analyzing cis- regulatory elements and trans-acting factors
• Drug screening
• siRNA and miRNA screening
• Multiplexed assays to study off-target effects
• Secretory pathway/protein localization reporter assays
• Signal transduction pathway analysis
• RNA splicing studies

The Cypridina luciferase is a 61kDa protein from the marine ostracod, Cypridina noctiluca. Cypridina luciferase is highly secreted into the cell culture media, allowing for live cell monitoring of reporter activity. Light output captured using a luminometer can be correlated with the amount of Cypridina luciferase protein produced and used to determine the activity of the promoter driving Cypridina expression. The Cypridina gene in Thermo Scientific Cypridina Luc Vectors is modified for expression in mammalian cells and provides optimal performance with the assay kit. The Cypridina glow reagent yields a Cypridina luciferase reaction with glow-type kinetics and is recommended for luminometers without injectors or for applications requiring batch processing of samples.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: 96-well plate, 384-well plate
Substrate: Vargulin (Cypridina Luciferin)
Technique: Enhanced Chemiluminescence
Assay Type: Reporter Enzyme, Luciferase Reporter Assay
Product Size: 100 reactions
Target Enzyme: Luciferase, Cypridina Luciferase
Substrate Type: Luciferase Substrate
Compatible Cells: Mammalian Cells
Detection Method: Bioluminescent
Substrate Properties: Chemical Substrate
For Use With (Equipment): Luminometer (Microplate)

Contents & storage

• Cypridina Glow Assay Buffer, 5 mL, store at 4°C
• Vargulin (1X), 5 µL, store at -20°C
• 2X Cell Lysis Buffer, 6 mL, store at room temperature

Upon receipt store kit at -20°C or store individual components as indicated above.

Documents

Manuals & protocols

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