Thermo Scientific™

Pierce™ Renilla-Firefly Luciferase Dual Assay Kit

Catalog number: 16186
Thermo Scientific™

Pierce™ Renilla-Firefly Luciferase Dual Assay Kit

Catalog number: 16186
Catalog Number
Unit Size
1000 reactions
Price (USD)
Catalog Number
Unit Size
100 reactions
Price (USD)
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
161861000 reactions
16185100 reactions
Product Overview
Citations & References
Additional Information
The Thermo Scientific Pierce Renilla-Firefly Luciferase Dual Assay Kit provides the necessary reagents to simultaneously detect intracellular Renilla and Red Firefly luciferase activity in mammalian whole cell lysates.

Features of the Renilla-Firefly Luciferase Dual Assay Kit:

Simultaneous—concurrent, filter-based, wavelength-separated detection of two luciferase activities
Sensitive—measure Green Renilla and red firefly luciferase activities in the same sample
Fast—no quenching step required, unlike in traditional sequential dual assays
Multiplex—capable of quantitating two cellular activities in the same sample(s)

The Pierce Renilla-Firefly Luciferase Dual Assay Kit is a highly sensitive assay that allows for the simultaneous detection of Green Renilla and Red Firefly luciferase activity. Green Renilla luciferase acts as an experimental reporter with constitutively active red firefly as a normalization control. As shown in the animation video below, this reporter-and-control combination enables simultaneous monitoring of experimental reporter and control luciferase activities in a single-read assay without the need for two-step addition of substrate reagents or quenching. The assay working solution contains substrates for both luciferases, and the reactions occur simultaneously with flash-type kinetics. The resulting luminescent signals are spectrally resolvable using filters. In a single sample, researchers can assay transcriptional activity of regulatory elements, signal transduction pathways, and effects of activators or inhibitors.

Cell lysis buffer, reaction buffer, and substrates coelenterazine and D-luciferin

Green Renilla and Red Firefly luciferase reporters; Filter sets at 525nm±20nm for Green Renilla Luc and 615 to 675nm for Red Firefly Luc. Luminometer or other instrument capable of monitoring luminescence, such as Thermo Scientific Luminoskan Ascent and Varioskan Flash Microplate Readers; Injector required for assessing more than 24 wells at a time.

• Study two regulatory elements at the same time
• Monitor two signaling pathways simultaneously
• Enable studying more than one target per screen (e.g. off-target effects)

Green Renilla produces brighter bioluminescent signal than firefly and native Renilla luciferases. The bioluminescent signal (λmax= 535nm) produced by Green Renilla luciferase protein results from the oxidation of coelenterazine. The second assay uses red firefly luciferase, which is a mutant form of the Japanese firefly luciferase Luciola cruciata. This luciferase produces a red-shifted emission spectrum (λmax= 613nm) that results from the oxidation of D-luciferin in the presence of ATP.

More Product Data
Simultaneous dual-emission detection of luciferase reporter assays

Related Products
Pierce™ Renilla-Firefly Luciferase Dual Assay Kit
For Research Use Only. Not for use in diagnostic procedures.


Assay Kit
Luciferase, Firefly Luciferase, Green Renilla Luciferase
Sufficient For
1000 Dual Luciferase Assays
1000 Reaction Kit
Reporter Enzyme, Luciferase Reporter Assay
Compatible Cells
Mammalian Cells
Detection Method
For Use With (Equipment)
Luminometer (Microplate)
384-well plate, 96-well plate
Label Type
Enzyme Labeled
Substrate Properties
Chemical Substrate
Enhanced Chemiluminescence
Substrate Type
Luciferase Substrate
Product Line
Coelenterazine, D-Luciferin

Contents & Storage

Sufficient For: 1000 dual-luciferase assays in microplate wells
• Renilla-Firefly Dual Assay Buffer, 50 mL (store at 4°C)
• 100X Coelenterazine, 0.5 mL (store at -80°C)
• D-Luciferin, 30 mg (store at 4°C)
• Luciferase Cell Lysis Buffer (2X), 60 mL (store at room temperature)

Upon receipt, store kit at -80°C, or store individual components as indicated above.


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