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Fötales Kälberserum, ultraniedriges IgG, Herkunftsland: USA
Gibco™

Fötales Kälberserum, ultraniedriges IgG, Herkunftsland: USA

Gibco™ fötale Kälberseren bieten einen ausgezeichneten Wert für die Zellkultur, die Spezialforschung und spezifische Assays. Sie erhalten das Vertrauen vonWeitere Informationen
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KatalognummerMengeFormat
16250078500 mLFlasche
A338190150 mLOne Shot
Katalognummer 16250078
Preis (EUR)
-
Menge:
500 mL
Format:
Flasche
Gibco™ fötale Kälberseren bieten einen ausgezeichneten Wert für die Zellkultur, die Spezialforschung und spezifische Assays. Sie erhalten das Vertrauen von Forschern mit gleichbleibender Qualität und prämierter Unterstützung, die Ihren Forschungsanforderungen und Ihrem Budget gerecht wird

Serum-Kategorie: Spezial
Herkunft: Vereinigte Staaten.
Die IgG-Konzentration liegt unter 5 µg/ml, und der BVD-Titer ist niedrig oder nicht nachweisbar.
Endotoxin-Gehalt: ≤50 EU/ml (routinemäßiger Spiegel ≤ 10 EU/ml).
Hämoglobin-Spiegel: ≤25 mg/dl.

Dieses Reagenz ist in mehreren Ländern als medizinisches Gerät klassifiziert. Wenn es in einer bestimmten Region nicht als medizinisches Gerät eingestuft wird, sollte es nur für den Laborgebrauch verwendet werden.
For research use or further manufacturing use only. Serum and blood proteins are not for direct administration into humans or animals.
Specifications
Versandbedingunggefroren
SpeziesRind/Bovine
AgeFötal
HerkunftslandVereinigte Staaten
FormFlüssig
FormatFlasche
ProdukttypFötales Kälberserum
Menge500 mL
Serum TreatmentVermindertes IgG
Unit SizeEach
Inhalt und Lagerung
Lagerbedingungen: ≤ -10° C
Versandbedingungen: Gefroren

Häufig gestellte Fragen (FAQ)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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