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Thermo Scientific™

PepMap™ 300 C18 HPLC Columns

Related applications:

Industrial Chromatography

This is the standard column for high-resolution, high-sensitivity protein identification for proteomics and biomarker discovery and verification. The Thermo Scientific™ Acclaim™ PepMap™ 300 C18 LC Columns is ideal for:

  • High-resolution peptide mapping of tryptic, natural, and synthetic peptides
  • LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology
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High loading capacity


Acclaim PepMap columns are exceptionally suitable for the analysis of low abundant peptides often found in complex proteomics samples.

The Acclaim PepMap C18 300Å column 5µm Particle Size is available in 150mm lengths in 75, 300, 1000, and classic 75µm I.D. to allow for tuning throughput, sensitivity, and amount of sample available.


  • Superior protein and peptide separations
  • Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity
  • Outstanding separation efficiency
  • Superior resolution and recovery
  • Easy installation with nanoViper™ Fingertight Fittings

Columns are available with Thermo Scientific™ Dionex™ nanoViper Fingertight Fittings:

  • Requires no tools
  • Provides virtually zero-dead-volume connections
  • Withstands pressures up to a 1000 bar
  • About Nano, capillary, and micro Acclaim PepMap columns

Nano (75µm), capillary (300µm) and micro (1000µm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and micro columns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and micro column in a variety of chemistries for peptides and protein analysis.

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.