Thermo Scientific™

PepMap™ 300 C18 HPLC Columns

Catalog number: 163574
Thermo Scientific™

PepMap™ 300 C18 HPLC Columns

Catalog number: 163574
This is the standard column for high-resolution, high-sensitivity protein identification for proteomics and biomarker discovery and verification. The Thermo Scientific™ Acclaim™ PepMap™ 300 C18 LC Columns is ideal for:

  • High-resolution peptide mapping of tryptic, natural, and synthetic peptides
  • LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology
 
Catalog Number
163574
Unit Size
Each
Particle Size
5 μm
Diameter (Metric)
0.075 mm
Length (Metric)
150 mm
Price (USD)
697.00
Your Price:
Availability
***
Full specifications
Quantity
Column FormatAnalytical Column
Diameter (Metric)0.075 mm
Flow Rate200 mL/min
Length (Metric)150 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size300 Å
Surface Area100 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load9%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
TypeAnalytical Column
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
5 μm
Diameter (Metric)
1 mm
Length (Metric)
150 mm
Price (USD)
945.00
Your Price:
Availability
***
Full specifications
Quantity
Column FormatAnalytical Column, NanoViper
Diameter (Metric)1 mm
Length (Metric)150 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size300 Å
Surface Area100 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load9%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
TypeAnalytical Column, NanoViper
Unit SizeEach
Catalog Number
Unit Size
Each
Particle Size
5 μm
Diameter (Metric)
0.3 mm
Length (Metric)
150 mm
Price (USD)
945.00
Your Price:
Availability
***
Full specifications
Quantity
Column FormatAnalytical Column, NanoViper
Diameter (Metric)0.3 mm
Length (Metric)150 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size300 Å
Surface Area100 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load9%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
TypeAnalytical Column, NanoViper
Unit SizeEach
Catalog Number
Unit Size
Pack of 5
Particle Size
5 μm
Diameter (Metric)
0.3 mm
Length (Metric)
5 mm
Price (USD)
454.00
Your Price:
Availability
***
Full specifications
Quantity
Column FormatTrap Cartridge
Diameter (Metric)0.3 mm
Length (Metric)5 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size300 Å
Surface Area100 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load9%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
TypeTrap Cartridge
Unit SizePack of 5
Catalog Number
Unit Size
Each
Particle Size
5 μm
Diameter (Metric)
0.075 mm
Length (Metric)
150 mm
Price (USD)
Availability
***
Full specifications
Quantity
-
Column FormatAnalytical Column, NanoViper
Diameter (Metric)0.075 mm
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)150 mm
Max. Pressure5800 psi (400 bar)
Particle Size5 μm
pH2 to 8
Pore Size300 Å
Surface Area150 m2/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load9%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
Unit SizeEach
Showing 5 of 5
Catalog NumberSpecificationsUnit SizeParticle SizeDiameter (Metric)Length (Metric)Price (USD)AvailabilityQuantity
163574Full specifications
Each5 μm0.075 mm150 mm
697.00
Your Price:
***
Column FormatAnalytical Column
Diameter (Metric)0.075 mm
Flow Rate200 mL/min
Length (Metric)150 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size300 Å
Surface Area100 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load9%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
TypeAnalytical Column
Unit SizeEach
164719Full specifications
Each5 μm1 mm150 mm
945.00
Your Price:
***
Column FormatAnalytical Column, NanoViper
Diameter (Metric)1 mm
Length (Metric)150 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size300 Å
Surface Area100 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load9%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
TypeAnalytical Column, NanoViper
Unit SizeEach
164718Full specifications
Each5 μm0.3 mm150 mm
945.00
Your Price:
***
Column FormatAnalytical Column, NanoViper
Diameter (Metric)0.3 mm
Length (Metric)150 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size300 Å
Surface Area100 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load9%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
TypeAnalytical Column, NanoViper
Unit SizeEach
163589Full specifications
Pack of 55 μm0.3 mm5 mm
454.00
Your Price:
***
Column FormatTrap Cartridge
Diameter (Metric)0.3 mm
Length (Metric)5 mm
Max. Pressure7250 psi (500 bar)
Particle Size5 μm
pH2 to 8
Pore Size300 Å
Surface Area100 m²/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load9%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
TypeTrap Cartridge
Unit SizePack of 5
164753Full specifications
Each5 μm0.075 mm150 mmContact Us ›***-
Column FormatAnalytical Column, NanoViper
Diameter (Metric)0.075 mm
For Use WithLC/MS, ESI-MS, MALDI-MS
Length (Metric)150 mm
Max. Pressure5800 psi (400 bar)
Particle Size5 μm
pH2 to 8
Pore Size300 Å
Surface Area150 m2/g
Temperature60°C
USP TypeL1
Stationary PhaseC18
Product LinenanoLC
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Carbon Load9%
Column TypeReversed Phase
EndcappedYes
PhaseReversed Phase
Unit SizeEach
Showing 5 of 5
High loading capacity

 

Acclaim PepMap columns are exceptionally suitable for the analysis of low abundant peptides often found in complex proteomics samples.

The Acclaim PepMap C18 300Å column 5µm Particle Size is available in 150mm lengths in 75, 300, 1000, and classic 75µm I.D. to allow for tuning throughput, sensitivity, and amount of sample available.

 

  • Superior protein and peptide separations
  • Trifluoroacetic acid (TFA)-free LC/MS minimizes suppression effects for enhanced MS sensitivity
  • Outstanding separation efficiency
  • Superior resolution and recovery
  • Easy installation with nanoViper™ Fingertight Fittings

Columns are available with Thermo Scientific™ Dionex™ nanoViper Fingertight Fittings:

  • Requires no tools
  • Provides virtually zero-dead-volume connections
  • Withstands pressures up to a 1000 bar
  • About Nano, capillary, and micro Acclaim PepMap columns

Nano (75µm), capillary (300µm) and micro (1000µm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and micro columns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and micro column in a variety of chemistries for peptides and protein analysis.

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

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Frequently asked questions (FAQs)

Citations & References