Thermo Scientific™ Related applications: Industrial ChromatographyGenerate high-resolution analyses of tryptic, natural, and synthetic peptides using Thermo Scientific™ Acclaim™ PepMap™ 100 C18 LC Columns. These columns are often used for LC-MS/MS peptide mapping for protein identification, biomarker discovery, and systems biology. Due to their high loading capacity, Acclaim PepMap 100 C18 columns are exceptionally suitable for the analysis of low abundant peptides in complex proteomics samples. Most of these C18 columns are pre-assembled with Thermo Scientific™ nanoViper™ Fingertight Fittings for easy installation. Classic columns are available as well.
|Catalog number||Product Size||Particle Size||Diameter (Metric)||Length (Metric)||Price (USD)|
|160434||Pack of 5||5µm||1mm||5mm|
|160454||Pack of 5||5µm||0.3mm||5mm|
|164197||Pack of 2||5µm||0.1mm||10mm|
|164213||Pack of 2||5µm||0.2mm||20mm|
|164199||Pack of 2||5µm||0.1mm||20mm|
|164535||Pack of 2||3µm||0.075mm||20mm|
|164564||Pack of 2||5µm||0.1m||20mm|
|164939||Each||2 µm||0.075 mm||750 mm|
PepMap nano columns are also available pre-assembled with Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fitting for a dead-volume-free connection to 1000 bar.
Nano (75µm), capillary (300µm) and micro (1000µm) columns are ideal for protein and peptide analyses. Sample amount is often limited and smaller I.D. columns can increase in sensitivity in sample-limited situations. While nano LC columns are often used in proteomics, the concentrating effects of capillary and micro columns are beneficial for metabolomics or for facilitating LC-MS interfacing. Thermo Scientific Acclaim PepMap columns are offered in nano, capillary, and micro column in a variety of chemistries for peptides and protein analysis.
Use PepMap Trap columns for desalting and preconcentration of peptides before LC separation with MS detection. The trap columns provide the highest efficiencies for one dimensional peptide mapping experiments and 2D-LC analyses.