Nunc™ 96-Well Optical-Bottom Microplate, white, TC Surface - FAQs

Q: Should I use an opaque black/white immuno plate, or an "optical-bottom" design?

Opaque plates can only be used on top reader instruments, which detect (or, in fluorescence mode, excite and detect) from above the plate. Readers that detect from underneath the plate (or imaging systems) use optical-bottom plates, which have a black or white upper well structure and a transparent (polystyrene film or optical coverglass) bottom. Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center .

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3 product FAQs found

What is the difference between the Nunclon Delta surface and a tissue culture (TC) surface from a competitor?

Our Nunclon Delta surface for adherent cells is a standard tissue culture (TC) surface modification that makes the polystyrene surface more hydrophilic. This facilitates maximum adhesion for a broad range of cell types.

The Nunclon Delta surface is Thermo Scientific Nunclon Delta-certified, which is a Thermo Fisher proprietary certification that has a quality standard to help ensure consistency. Every lot of Thermo Scientific Nunclon Delta-certified cell cultureware is tested with Gibco media for:
- Less than 10% growth variation in L929, Hel299 and primary chick embryo cells
- Less than 15% variation colony formation of V79-4 cells

More information and all applicable products can be found in the "Product selection guide for every step of your cell culture workflow" brochure .

How does cell culturing on Nunc 384-Well Microplates that have a cell culture-treated surface compare with 96-Well Microplates that have a cell culture-treated surface?

The cell culture-treated surfaces are very similar but the wells of the Nunc 384-Well Microplates are smaller than the wells of the Nunc 96-Well Microplate.
Culture recommendations for the Nunc 384-Well Microplates are:
- 15 - 50 µL media per well
- 0.5 - 2.0 x 10^4 cells per well

Should I use an opaque black/white immuno plate, or an "optical-bottom" design?

Opaque plates can only be used on “top reader” instruments, which detect (or, in fluorescence mode, excite and detect) from above the plate. Readers that detect from underneath the plate (or imaging systems) use optical-bottom plates, which have a black or white upper well structure and a transparent (polystyrene film or optical coverglass) bottom.

Find additional tips, troubleshooting help, and resources within our Antibodies and Immunoassays Support Center.