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Citações e referências (3)
Gibco™
McCoy's 5A (Modified) Medium
McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, establishedLeia mais
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| Número do catálogo | Quantity |
|---|---|
| 16600082 | 500 mL |
| 16600108 | 10 x 500 mL |
Número do catálogo 16600082
Preço (BRL)
262,94
Each
Quantity:
500 mL
Preço (BRL)
262,94
Each
McCoy's 5A (modified) Medium is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
This McCoy's 5A is modified as follows:
| With | Without |
| • High Glucose | • Sodium Pyruvate |
| • L-glutamine | • HEPES |
| • Bacto-peptone | |
| • Phenol Red |
The complete formulation is available.
Using McCoy's 5A (modified) Medium
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Especificações
Cell LineRat fibroblasts
Cell TypeBiopsy Tissues
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco
Product TypeMcCoy's 5A Modified Medium
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red, Sodium Bicarbonate
Without AdditivesNo HEPES, No Sodium Pyruvate
Unit SizeEach
Conteúdo e armazenamento
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Citações e referências (3)
Citações e referências
Abstract
Novel kelch-like protein, KLEIP, is involved in actin assembly at cell-cell contact sites of Madin-Darby canine kidney cells.
Journal:Mol Biol Cell
PubMed ID:14668487
Dynamic rearrangements of cell-cell adhesion underlie a diverse range of physiological processes, but their precise molecular mechanisms are still obscure. Thus, identification of novel players that are involved in cell-cell adhesion would be important. We isolated a human kelch-related protein, Kelch-like ECT2 interacting protein (KLEIP), which contains the broad-complex, tramtrack,
DMH1, a novel BMP small molecule inhibitor, increases cardiomyocyte progenitors and promotes cardiac differentiation in mouse embryonic stem cells.
Journal:PLoS One
PubMed ID:22848549
The possibility of using cell-based therapeutics to treat cardiac failure has generated significant interest since the initial introduction of stem cell-based technologies. However, the methods to quickly and robustly direct stem cell differentiation towards cardiac cell types have been limited by a reliance on recombinant growth factors to provide necessary
A 2-D liquid separations/mass mapping method for interlysate comparison of ovarian cancers.
Journal:Anal Chem
PubMed ID:11985308
A two-dimensional liquid phase separation of proteins from whole cell lysates coupled on-line to an electrospray-ionization time-of-flight (ESI-TOF) mass spectrometer (MS) is used to map the protein content of ovarian surface epithelial cells (OSE) and an ovarian carcinoma-derived cell line (ES2). The two dimensions involve the use of liquid isoelectric