Keratinocyte SFM (1X) Kit

Keratinocyte SFM (serum-free medium) is optimized for the growth and maintenance of human keratinocytes and other types of epithelial cells詳細を見る

製品番号（カタログ番号）	数量
17005042	500 mL

製品番号（カタログ番号） 17005042

価格（JPY）

30,800

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500 mL

Keratinocyte SFM (serum-free medium) is optimized for the growth and maintenance of human keratinocytes and other types of epithelial cells without the need for a feeder layer of cells. The complete medium may be used to cultivate human epidermal keratinocytes for studies involving dermal substitutes, in vitro toxicology, and gene therapy.

Keratinocyte SFM is a complete serum-free medium when supplemented with human recombinant epidermal growth factor (rEGF) and bovine pituitary extract (BPE) at the time of use. Both supplements, rEGF and BPE, are supplied in separate packaging with the basal medium.

研究用途にのみご使用ください。診断目的には使用できません。

細胞タイプKeratinocyte Cells, Epithelial Cells

濃度1 X

製品タイプKit

数量500 mL

品質保持期間12 Months

分類Serum-free

Culture TypeMammalian Cell Culture

形状Liquid

Serum LevelSerum-free

無菌性Sterile-filtered

Sterilization MethodSterile-filtered

添加剤ありGlutamine, Phenol Red

Unit SizeEach

組成および保存条件

• 500 mL Keratinocyte-SFM Basal Medium; store in refrigerator (2–8°C) and protect from light
• 25 mg Bovine Pituitary Extract (BPE); store in freezer (-5 to -20°C) and protect from light
• 2.5 μg EGF, Human Recombinant; store in freezer (-5 to -20°C) and protect from light

Complete Medium: store in refrigerator (2–8°C) and protect from light

よくあるご質問（FAQ）

Are the Keratinocyte SFM basal medium and supplement kit available separately?

The basal medium is not available for purchase individually, and can only be ordered as part of the Keratinocyte SFM kit (catalog # 17005-042). The supplements are available separately: Bovine Pituitary Extract is catalog # 13028-014 and the EGF is catalog # PHG0311.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture mouse keratinocytes?

The Keratinocyte SFM without calcium chloride (Cat. No. 37010-022) is appropriate for the culture of mouse keratinocytes. The medium should be supplemented with calcium chloride at a final concentration of 0.02 mM.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which serum-free media can be used to culture human keratinocytes?

There are a variety of options for serum-free culture of human keratinocytes. Keratinocyte SFM (Cat. No. 17005-042) is a widely used kit that includes the basal medium, bovine pituitary extract (BPE) and Epidermal Growth Factor (EGF). If you would like a serum-free, defined culture system, the options are the Defined Keratinocyte SFM kit (Cat. No. 10744-019) or Epilife medium (Cat. No. M-EPI-500-CA) supplemented with EDGS (Cat. No. S-012-5). If you would like an animal origin free defined system, Epilife medium (Cat. No. M-EPI-500-CA) with S7 supplement (Cat. No. S-017-5) is an excellent choice.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the calcium concentration in Keratinocyte SFM (1X) (Cat. No. 17005042)?

Keratinocyte SFM (1X) (Cat. No. 17005042) includes Keratinocyte-SFM (Cat. No. 10724011) and Keratinocytes Supplements (Cat. No. 37000015), which contains Bovine Pituitary Extract (BPE) EGF, Human Recombinant. The complete media is prepared by adding the supplements to the base media. The Keratinocyte-SFM (Cat. No. 10724011) contains calcium chloride at 12.98 mg/L and D calcium pantothenate at 0.29 mg/L.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For how long can I store Keratinocyte-SFM at 4 degrees C?

The base Keratinocyte-SFM has a shelf life of 12 months when stored at 2 to 8 degrees C, protected from light. Keratinocyte-SFM Supplement has a shelf life of 18 months when stored at -20 to 5 degrees C. The complete media should not be stored for longer than 4 weeks at 4 degrees C

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.