North2South™ Chemiluminescent Hybridization and Detection Kit

Catalog number: 17097

Thermo Scientific™  Related applications: Nucleic Acid Gel Electrophoresis & Blotting

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Thermo Scientific North2South Chemiluminescent Detection Kit combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.

Features of the North2South Chemiluminescent Detection Kit:

• Sensitivity equal to or greater than 32P
• Much faster than DIG detection systems
• Amenable to nearly any DNA or RNA hybridization method
• Optimized hybridization and blocking buffers and protocol steps yield consistent results from experiment to experiment
• High level of light output and consistent low background allows for very short and multiple exposure times using cooled CCD camera systems or film
• Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user
• Specific plaques can be picked out of areas that are very dense with signal due to extremely low background

The North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin- HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.

Related Products
North2South™ Complete Biotin Random Prime Labeling and Detection Kit
North2South™ Chemiluminescent Substrate for HRP
North2South™ Hybridization Buffer
North2South™ Hybridization Stringency Wash Buffer (2X)
For Research Use Only. Not for use in diagnostic procedures.


Product Size: 10 blots

Contents & storage

Sufficient For: 1000 cm2 of membrane (10 mini-gel blots)
• Stabilized Streptavidin-HRP, 1.5 mL
• Luminol/Enhancer Solution, 80 mL
• Stable Peroxide Solution, 80 mL
• Hybridization Buffer, 125 mL
• Hybridization Stringency Wash Buffer (2X), 375 mL
• Blocking Buffer, 500 mL
• Wash Buffer (4X), 500 mL
• Substrate Equilibration Buffer, 500 mL

Upon receipt, store kit at 4°C.


Manuals & protocols

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