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Gibco™

Collagenase, Type IV, powder

Catalog number:  17104019

Related applications:

Mammalian Cell Culture

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Description

Collagenase is a protease that cleaves the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Glyc-Pro, which is found with high frequency in collagen. Collagenase is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone. Collagenase disaggregation is suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium. Collagenase is relatively gentle, dissociates well at physiological temperature and pH, and requires no mechanical agitation or special equipment.

Gibco® Collagenase Type IV is isolated from Clostridium histolyticum and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation and organ perfusions. Gibco® Collagenase Type IV activity is guaranteed to be greater than 160 units/mg. Compared to other collagenase preparations, Gibco® Collagenase Type IV has low tryptic activity and is well-suited for the digestion of islet cells from the pancreas.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Shipping Condition: Room Temperature
Phenol Red Indicator: No Phenol Red
Reagent Type: Collagenase

Contents & storage

Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture