The Thermo Scientific™ North2South™ Biotin Random Prime Labeling Kit provides the components and procedure to successfully prepare biotin-labeled DNA probes for use in Southern blotting and other DNA hybridization methods involving streptavidin detection systems.
This kit includes the North2South Chemiluminescent Detection Kit, which combines a novel ECL substrate for horseradish peroxidase (HRP) and optimized hybridization and blocking buffers to ensure consistent, high-sensitivity Southern blot results.
Features of the North2South Chemiluminescent Detection Kit:
• Sensitivity equal to or greater than 32P • Much faster than DIG detection systems • Amenable to nearly any DNA or RNA hybridization method • Optimized hybridization and blocking buffers and protocol steps yield consistent results from experiment to experiment • High level of light output and consistent low background allows for very short and multiple exposure times using cooled CCD camera systems or film • Ready-to-use buffers and short process time makes switching from radioactive detection to chemiluminescent detection an easy transition, even for the novice user • Specific plaques can be picked out of areas that are very dense with signal due to extremely low background
The North2South Hybridization and Detection Kit combines a novel enhanced luminol substrate for horseradish peroxidase (HRP) with optimized pre-made hybridization and blocking buffers to ensure consistent results and high sensitivity. Any nucleic acid blot can be hybridized with the appropriate biotinylated (biotin-labeled) oligonucleotide probe. After a brief membrane blocking step, the biotin label is detected with streptavidin- HRP and a 5-minute incubation with the chemiluminescent substrate. Results are easily recorded by brief exposure to X-ray film (1-15 minutes) or CCD camera.