Increase cell survival by more than 50% with new Gibco™ B-27™ Plus Neuronal Culture System.

N-2 Supplement (100X)

Catalog number:  17502048

Gibco™  Related applications: Mammalian Cell Culture | Primary Cell Culture | Stem Cell Culture

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N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein’s N-1 formulation. N-2 Supplement is intended for use with Neurobasal® media.

N-2 Supplement is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS). N-2 Supplement can be used as a substitute for Bottenstein’s N-1 formulation. N-2 Supplement is provided as a 100X concentrate.

N-2 Supplement may be used with Neurobasal® media supplemented with growth factors such as bFGF and EGF or used with DMEM.

Learn more about N-2 Supplement formulation.

Learn more about neural cell culture products from Invitrogen at

For Research Use Only. Not intended for any animal or human therapeutic of diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.


Cell Type: Neuronal Cells, Neuronal Tumor Cells
Classification: Serum-Free
Product Size: 5 mL
Purity or Quality Grade: Cell Culture Grade
Shipping Condition: Dry Ice

Contents & storage

Store in freezer (-5 to -30°C).

Media formulations


Manuals & protocols

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