Gibco™

Supplément (50X) B-27™, sans sérum

Name: Supplément (50X) B-27™, sans sérum
SKU: 17504001
Price: 1172.00 EUR

Le supplément de culture cellulaire neuronale le plus cité, le supplément B-27, est un supplément optimisé sans sérum utilisé pourAfficher plus

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Référence	Quantité
17504001	100 mL
17504044	10 ml

2 options

Référence 17504001

Prix (EUR)

1 172,00

Each

Quantité:

100 mL

Prix (EUR)

1 172,00

Each

Le supplément de culture cellulaire neuronale le plus cité, le supplément B-27, est un supplément optimisé sans sérum utilisé pour faciliter la croissance à faible ou haute densité et la viabilité à court ou long terme des neurones embryonnaires, post-natals et adultes hippocampiques et autres neurones du SNC. Le supplément B-27 est fourni sous forme de liquide 50 X et doit être utilisé avec un milieu Neurobasal ou Neurobasal-A pour la culture de cellules neuronales sans nécessiter de couche nourricière d’astrocytes.

Le supplément B-27 convient à la plupart des applications relatives aux cellules neuronales. Pour d’autres applications, nous fournissons des formulations alternatives de suppléments B-27 et de milieux Neurobasal.

Pour les protocoles établis à l’aide du supplément B-27, consultez notre manuel sur le protocole de neurobiologie Gibco.

Consultez les publications à l’aide des produits de neurobiologie Gibco chez notre Gibco CellCite.

For Research Use Only. Not for use in diagnostic procedures.

Spécifications

Type de celluleCellules neurales

Concentration10X

Type de cultureCulture de cellules de mammifères

Quantité100 mL

Niveau de sérumSans sérum

Durée de conservation12 mois

Testé pourEndotoxine, performances, stérilité, mycoplasmes

ClassificationSans sérum

FormeLiquide

Type de produitSupplément de culture cellulaire

StérilitéStérile

Unit SizeEach

Contenu et stockage

Conserver au congélateur (-5 à -20°C) à l’abri de la lumière.

Foire aux questions (FAQ)

Can B-27 supplement be filtered?

Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can Neurobasal Medium alone or Neurobasal Medium supplemented with B-27 supplement be frozen to extend product shelf life?

No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which media system should I use for primary culture of P0- or P1-derived mixed hippocampal cells?

We recommend using Neurobasal Plus Medium with B-27 Plus Supplement for long-term culture of mixed hippocampal cells. For short-term culture of a pure population of hippocampal neurons, we would recommend using Neurobasal-A Medium with B-27 Supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer B-27 supplements with and without insulin?

Yes, we do offer B-27 Supplement, minus insulin (Cat. Nos. A1895601 and A1895602). All our other B-27 supplements do contain insulin.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Supplément (50X) B-27™, sans sérum FAQs