Nunc™ Lab-Tek™ Chamber Slide System

Unfortunately, we do not have a recommended cell density for our LabTek Chamber Slides. However, you can refer to the following link, which provides recommended cell densities for our other cell culture products:
https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/cell-culture-useful-numbers.html

Please review the following key differences between the Nunc Lab-Tek Chamber Slide System and the Nunc Lab-Tek II Chamber Slide System:
- The Nunc Lab-Tek Chamber Slide System is available in Permanox plastic or glass and incorporates a silicone gasket.
- The Nunc Lab-Tek II Chamber Slide System is available in glass only and do not have a silicone gasket.
- The Nunc Lab-Tek II Chamber Slide System has a blue hydrophobic border and the upper structure is attached by an adhesive. Supplied with this product is a key and holder system which is used to remove the upper structure from the glass slide.
- The Nunc Lab-Tek Chamber Slide System is available in 1-, 2-, 4-, 8- or 16-well formats.
- The Nunc Lab-Tek II Chamber Slide System is available in 1-, 2-, 4- or 8-well formats.

Our Nunc Lab-Tek Chamber Slide products are used for fluorescence analyses at the microscopic level.
The following are commonly used excitation and emittance wavelengths:
FITC
- Excitation: 420-490 nm
- Emittance: 520-550 nm
Rhodamine
- Excitation: 510-560 nm
- Emittance: 570-610 nm
Hoechst
- Excitation: 330-380 nm
- Emittance: 480-510 nm

We have a Technical Bulletin (link below) describing fixation reagents that are compatible with Permanox plastic: https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/D19581.pdf

The Nunc Lab-Tek Chamber Slide System products, made of soda lime glass, are not coated with any chemicals or biological reagents. Since glass is typically a good substrate for adherent cells, these soda lime glass products are not surface modified in any manner.