Invitrogen™

T4 DNA Polymerase

Catalog number: 18005017
Invitrogen™

T4 DNA Polymerase

Catalog number: 18005017
Catalog Number
18005017
also known as 18005-017
Unit Size
50 units
Price (USD)
122.00
Your Price:
Availability
***
Quantity
Catalog Number
18005025
also known as 18005-025
Unit Size
250 units
Price (USD)
379.00
Your Price:
Availability
***
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
18005017
also known as 18005-017
50 units
122.00
Your Price:
***
18005025
also known as 18005-025
250 units
379.00
Your Price:
***
Product Overview
Figures
Videos
Documents
FAQ
Citations & References
Additional Information
Recommendations
T4 DNA Polymerase is a DNA polymerase that has a 3´-exodeoxyribonuclease activity, but lacks 5´a3´ exodeoxyribonuclease activity. A T4 DNA Polymerase Technical Bulletin is available.

Applications:
Labeling double-stranded linear DNA by replacement synthesis (1). Oligonucleotide-directed, site-specific mutagenesis (2). 3´ end-labeling of double-stranded DNA (3). Polishing both 5´ or 3 overhangs to make blunt ends (4).

Source:
Purified from E. coli expressing the T4 DNA Polymerase gene on a plasmid.

Performance and Quality Testing:
Single- and double-stranded endodeoxyribonuclease and phosphatase assays; exodeoxyribonuclease and polymerase activities tested.

Unit Definition:
One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using DNase I-nicked DNA as template•primer.

Unit Reaction Conditions:
50 mM glycine-NaOH (pH 8.8), 16.6 mM (NH4)2SO4 , 6 mM MgCl2 , 6.5 µM EDTA, 10 mM 2-mercaptoethanol, 0.165 mg/ml BSA, 1.6 mg/ml DNase I-nicked salmon testes DNA, 0.33 mM dCTP, 0.33 mM dATP, 0.33 mM dGTP, 0.33 mM dTTP, 76 nM [3H]dTTP, and enzyme in 0.1 ml for 30 min. at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Exonuclease Activity
3' - 5'
Hot Start
No
Overhang
Blunt
Polymerase
T4 DNA Polymerase
Shipping Condition
Wet Ice
Form
Liquid
Product Type
DNA Polymerase
Quantity
50 units

Contents & Storage

T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165 mM Tris acetate (pH 7.9), 330 mM sodium acetate, 50 mM magnesium acetate, 5 mM DTT]. Store at -20°C.

Figures

Frequently asked questions (FAQs)

Citations & References