T4 DNA Polymerase is a DNA polymerase that has a 3´-exodeoxyribonuclease activity, but lacks 5´a3´ exodeoxyribonuclease activity. A T4 DNA Polymerase Technical Bulletin is available. Applications: Labeling double-stranded linear DNA by replacement synthesis (1). Oligonucleotide-directed, site-specific mutagenesis (2). 3´ end-labeling of double-stranded DNA (3). Polishing both 5´ or 3 overhangs to make blunt ends (4). Source: Purified from E. coli expressing the T4 DNA Polymerase gene on a plasmid. Performance and Quality Testing: Single- and double-stranded endodeoxyribonuclease and phosphatase assays; exodeoxyribonuclease and polymerase activities tested. Unit Definition: One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min. at 37°C using DNase I-nicked DNA as template•primer. Unit Reaction Conditions: 50 mM glycine-NaOH (pH 8.8), 16.6 mM (NH4)2SO4 , 6 mM MgCl2 , 6.5 µM EDTA, 10 mM 2-mercaptoethanol, 0.165 mg/ml BSA, 1.6 mg/ml DNase I-nicked salmon testes DNA, 0.33 mM dCTP, 0.33 mM dATP, 0.33 mM dGTP, 0.33 mM dTTP, 76 nM [3H]dTTP, and enzyme in 0.1 ml for 30 min. at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
3' - 5'
T4 DNA Polymerase
Contents & storage
T4 DNA Polymerase is supplied with a vial of 5X T4 DNA Polymerase buffer [165 mM Tris acetate (pH 7.9), 330 mM sodium acetate, 50 mM magnesium acetate, 5 mM DTT]. Store at -20°C.