Invitrogen™

CIAP (Calf Intestinal Alkaline Phosphatase), 20 U/µL

Catalog number:  18009019

Related applications:

Restriction Enzyme Cloning

Back to top

Description

Calf Intestinal Alkaline Phosphatase (CIAP) is a phosphomonoesterase that removes 3´ and 5´ phosphates from DNA and RNA.

Concentration: 20 units/µL

Applications:
Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation (1). Dephosphorylation of 5´ termini of nucleic acids prior to forward reaction with kinase.

Source: Purified from calf intestinal mucosa.

Performance and Quality Testing:
Endodeoxyribonuclease, 3´ exodeoxyribonuclease, and ribonuclease assays; dephosphorylation efficiency measured in a transformation assay.

Unit Definition:
One unit hydrolyzes 1 µmol of 4-nitrophenyl phosphate in 1 min. at 37°C.

Unit Reaction Conditions:
1 M diethanolamine buffer, 10 mM 4-nitrophenyl phosphate, 0.25 mM MgCl2 (pH 9.8) in 900 µl for 10 min. at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Shipping Condition: Approved for shipment on Wet or Dry Ice
Enzyme: CIP
Concentration: 20U/μL

Contents & storage

Calf Intestinal Alkaline Phosphatase (20 units/µL) is supplied with a vial of 10X dephosphorylation buffer [500 mM Tris-HCl (pH 8.5), 1 mM EDTA], vial of dilution buffer [50% glycerol, 25 mM Tris-HCl (pH 7.6), 1 mM MgCl2 , and 0.1 mM ZnCl2 ]. Store Calf Intestinal Alkaline Phosphatase at -20°C.