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DNA Polymerase I
DNA Polymerase I is a DNA polymerase with 5´→3´ and 3´→5´ exodeoxyribonuclease activities. DNA Polymerase I also incorporates biotinylated nucleotides.•Read more
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| Catalog Number | Quantity |
|---|---|
| 18010025 | 1000 U |
| 18010017 | 250 U |
Catalog number 18010025
Price (USD)/ Each
506.65
Online Exclusive
606.00Save 99.35 (16%)
Quantity:
1000 U
Price (USD)/ Each
506.65
Online Exclusive
606.00Save 99.35 (16%)
DNA Polymerase I is a DNA polymerase with 5´→3´ and 3´→5´ exodeoxyribonuclease activities. DNA Polymerase I also incorporates biotinylated nucleotides.
• Applications—DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs
• Source—purified from E. coli expressing the DNA Polymerase I gene on a plasmid
Performance and quality testing
Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.
Unit definition
One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.
Unit reaction conditions
50 mM potassium phosphate (pH 7.0), 6.7 mM MgCl2 , 1 mM 2-mercaptoethanol, 80 μg/mL template/primer, 32 μM dTTP, 69 nM [3H]dTTP, and enzyme in 100 μL for 30 min at 37°C.
• Applications—DNase I-dependent nick translation, second-strand synthesis in cDNA cloning, fill-in of 5´ overhangs
• Source—purified from E. coli expressing the DNA Polymerase I gene on a plasmid
Performance and quality testing
Endodeoxyribonuclease assay; efficiency of DNase I-dependent nick translation determined.
Unit definition
One unit incorporates 10 nmol of total deoxyribonucleotide into acid-precipitable material in 30 min at 37°C using template/primer.
Unit reaction conditions
50 mM potassium phosphate (pH 7.0), 6.7 mM MgCl2 , 1 mM 2-mercaptoethanol, 80 μg/mL template/primer, 32 μM dTTP, 69 nM [3H]dTTP, and enzyme in 100 μL for 30 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionDNA Polymerase
PolymeraseDNA Polymerase I
Exonuclease Activity3' - 5', 5' - 3'
Quantity1000 U
Shipping ConditionWet Ice
Hot StartNo
Unit SizeEach
Contents & Storage
1000 units of DNA polymerase in a storage buffer of 50 mM potassium phosphate (pH 7.0), 100 mM KCl, 1 mM DTT, and 50% (v/v) glycerol. It is supplied at 5–10 units/μl.
Store at -5°C to -30°C.
Store at -5°C to -30°C.