Invitrogen™

Bacterial Alkaline Phosphatase

Catalog number:  18011015

Related applications:

Restriction Enzyme Cloning

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Description

Bacterial Alkaline Phosphatase (BAP) removes 3´ and 5´ phosphates from DNA and RNA. BAP is active at 65°C for at least 1 h and is inactivated by phenol extraction.

Applications:
Dephosphorylation of 5´-phosphorylated termini of vector DNA to prevent self-ligation. Dephosphorylation of 5´ termini of nucleic acids prior to forward reaction with kinase.

Source:
Purified from E. coli C90.

Performance and Quality Testing:
Endodeoxyribonuclease, exodeoxyribonuclease, and ribonuclease assays; dephosphorylation
efficiency determined.

Unit Definition:
One unit hydrolyzes 1 nmol of ATP in 30 min. at 37°C.

Unit Reaction Conditions:
10 mM Tris-HCl (pH 8.0), 1.5 mM [γ-32P]ATP, and enzyme in 50 µl for 30 min. at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Shipping Condition: Wet Ice
Enzyme: BAP

Contents & storage

Bacterial Alkaline Phosphatase is supplied with a vial of 10X dephosphorylation buffer [100 mM Tris-HCl (pH 8.0)]. Store at -20°C.