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Citations & References (6)
Invitrogen™
Ribonuclease H
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand of an RNA-DNA hybrid to produce 5´Read more
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| Catalog Number | Quantity |
|---|---|
| 18021014 | 30 U |
| 18021071 | 120 U |
Catalog number 18021014
Price (KRW)
261,000
Online offer
Ends: 31-Dec-2025
289,000Save 28,000 (10%)
Each
Quantity:
30 U
Price (KRW)
261,000
Online offer
Ends: 31-Dec-2025
289,000Save 28,000 (10%)
Each
Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand of an RNA-DNA hybrid to produce 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA.
Applications
Removal of mRNA during second-strand cDNA synthesis. Removal of poly(A) sequences from mRNA in the presence of oligo(dT). Oligodeoxyribonucleotide-directed cleavage of RNA.
Source
Purified from E. coli expressing the E. coli RNase H gene on a plasmid.
Performance and quality testing
Ribonuclease, nonspecific endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease.
Unit definition
One unit hydrolyzes 1 nmol of RNA in 3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min at 37°C.
Unit reaction conditions
20 mM Tris-HCl (pH 7.5), 0.1 M KCl, 10 mM MgCl2 , 0.1 mM DTT, 5% (w/v) sucrose, 0.5 nmol 3H-labeled poly(A)poly(dT), and enzyme in 50 μL for 20 min at 37°C.
Applications
Removal of mRNA during second-strand cDNA synthesis. Removal of poly(A) sequences from mRNA in the presence of oligo(dT). Oligodeoxyribonucleotide-directed cleavage of RNA.
Source
Purified from E. coli expressing the E. coli RNase H gene on a plasmid.
Performance and quality testing
Ribonuclease, nonspecific endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease.
Unit definition
One unit hydrolyzes 1 nmol of RNA in 3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min at 37°C.
Unit reaction conditions
20 mM Tris-HCl (pH 7.5), 0.1 M KCl, 10 mM MgCl2 , 0.1 mM DTT, 5% (w/v) sucrose, 0.5 nmol 3H-labeled poly(A)poly(dT), and enzyme in 50 μL for 20 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeRibonuclease H
Quantity30 U
Shipping ConditionApproved for shipment on Wet or Dry Ice
Concentration2 U/μL
EnzymeRNase
Unit SizeEach
Contents & Storage
1 vial of Ribonuclease H containing 30 units supplied at a concentration of 2U/μl
Store at -20°C in a non-frost-free freezer.
Store at -20°C in a non-frost-free freezer.
Frequently asked questions (FAQs)
Do you offer RNAse H from the SuperScript III First Strand Synthesis System as a stand-alone item?
Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?
Citations & References (6)
Citations & References
Abstract
Functions of Transforming Growth Factor-beta Family Type I Receptors and Smad Proteins in the Hypertrophic Maturation and Osteoblastic Differentiation of Chondrocytes.
Journal:J Biol Chem
PubMed ID:12082094
'We investigated the effects of bone morphogenetic protein (BMP)-2, a member of the transforming growth factor-beta superfamily, on the regulation of the chondrocyte phenotype, and we identified signaling molecules involved in this regulation. BMP-2 triggers three concomitant responses in mouse primary chondrocytes and chondrocytic MC615 cells. First, BMP-2 stimulates expression
De novo ceramide regulates the alternative splicing of caspase 9 and Bcl-x in A549 lung adenocarcinoma cells. Dependence on protein phosphatase-1.
Journal:J Biol Chem
PubMed ID:11801602
'In a recent study, we showed that ceramide induces the dephosphorylation of SR proteins, a family of protein factors that regulate alternative splicing. In this study, the regulation of the alternative processing of pre-mRNA of both caspase 9 and Bcl-x(L) was examined in response to ceramide. Treatment of A549 lung
The novel WD-repeat protein MORG1 acts as a molecular scaffold for HIF prolyl-hydroxylase 3 (PHD3).
Journal:J Biol Chem
PubMed ID:16407229
'Hypoxia-inducible factor-1 (HIF-1), a transcriptional complex composed of an oxygen-sensitive alpha- and a beta-subunit, plays a pivotal role in cellular adaptation to low oxygen availability. Under normoxia, the alpha-subunit of HIF-1 is hydroxylated by a family of prolyl hydroxylases (PHDs) and consequently targeted for proteasomal degradation. Three different prolyl hydroxylases
High-sensitivity detection of DNA hybridization on microarrays using resonance light scattering.
Journal:Anal Chem
PubMed ID:11985309
The application of resonance light scattering (RLS) particles for high-sensitivity detection of DNA hybridization on cDNA microarrays is demonstrated. Arrays composed of approximately 2000 human genes (
mRNA decay is rapidly induced after spore germination of Saccharomyces cerevisiae.
Journal:J Biol Chem
PubMed ID:12181322
Spores from the yeast Saccharomyces cerevisiae can germinate and resume their vegetative growth when placed in favorable conditions. Biochemical studies on germination have been limited by the difficulty of obtaining a pure population of spores germinating synchronously. Here, we report that spores can be purified and sorted according to their