Invitrogen™

Ribonuclease H

Catalog number:  18021071

Related applications:

Reverse Transcription

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Description

Ribonuclease H (RNase H) is an endoribonuclease that specifically degrades the RNA strand of an RNA-DNA hybrid to produce 5´ phosphate-terminated oligoribonucleotides and single-stranded DNA.

Applications

Removal of mRNA during second-strand cDNA synthesis. Removal of poly(A) sequences from mRNA in the presence of oligo(dT). Oligodeoxyribonucleotide-directed cleavage of RNA.

Source

Purified from E. coli expressing the E. coli RNase H gene on a plasmid.

Performance and quality testing

Ribonuclease, nonspecific endodeoxyribonuclease, 3´ and 5´ exodeoxyribonuclease.

Unit definition

One unit hydrolyzes 1 nmol of RNA in 3H-labeled poly(A)poly(dT) to acid-soluble material in 20 min at 37°C.

Unit reaction conditions

20 mM Tris-HCl (pH 7.5), 0.1 M KCl, 10 mM MgCl2 , 0.1 mM DTT, 5% (w/v) sucrose, 0.5 nmol 3H-labeled poly(A)poly(dT), and enzyme in 50 µL for 20 min at 37°C.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Quantity: 120U
Shipping Condition: Approved for shipment on Wet or Dry Ice
Enzyme: RNase

Contents & storage

4 vials of Ribonuclease H with each containing 30 units supplied at a concentration of 2U/µl

Store at -20°C in a non-frost-free freezer.