For PCR enzyme with ≤0.01 copy of bacterial gDNA per enzyme unit, check out Invitrogen Platinum Taq DNA Polymerase, DNA-free.
Invitrogen™

Taq DNA Polymerase, native

Catalog number:  18038018

Related applications:

PCR

| Chromatin Biology

| Methylation Analysis

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Description

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity (see figure). With Taq DNA Polymerase, you get:

  • Your choice of recombinant or native enzyme
  • Amplification of PCR products up to 5 kb in size
  • An enzyme that is licensed and qualified for PCR

    Applications
    Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing

    Source
    Native enzyme is purified from Thermus aquaticus YT1.

    Unit definition
    One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 min at 74°C.
  • For Research Use Only. Not for use in diagnostic procedures.

    Specifications

    Exonuclease Activity: 5' - 3'
    Hot Start: No
    Quantity: 100 units
    Shipping Condition: Approved for shipment on Wet or Dry Ice
    Fidelity (vs. Taq): 1 X
    GC-Rich PCR Performance: Low
    Overhang: 3'-A
    Polymerase: Taq DNA Polymerase
    Reaction Format: Separate Components
    Reaction Speed: Standard
    Size (Final Product): 5 kb or less

    Contents & storage

    Contains:
    • 20 µl Taq DNA Polymerase (5 U/µl)
    • 1.25 ml 10X PCR buffer (without magnesium)
    • 1 ml Magnesium Chloride (50 mM)

    Store at -20°C in a non-frost-free freezer. Stable for 6 months when stored properly.