Taq DNA Polymerase, native
<i>Taq</i> DNA Polymerase, native
Citas y referencias (6)
Invitrogen™

Taq DNA Polymerase, native

La ADN polimerasa Taq es una enzima termoestable que sintetiza el ADN a partir de moldes monocatenarios en presencia deMás información
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Número de catálogoCantidad
18038042500 unidades
18038018100 unidades
180380673 x 500 unidades
180382405000 unidades
Número de catálogo 18038042
Precio (CLP)
415.800
Each
Añadir al carro de la compra
Cantidad:
500 unidades
Pedido a granel o personalizado
Precio (CLP)
415.800
Each
Añadir al carro de la compra
La ADN polimerasa Taq es una enzima termoestable que sintetiza el ADN a partir de moldes monocatenarios en presencia de dNTP y un cebador. La enzima se compone de un polipéptido único con un peso molecular de 94 kDa. Tiene una actividad de ADN polimerasa de 5´→3´ y una actividad de exonucleasa de 5´→3´ (consulte la figura). Con la ADN polimerasa Taq, obtendrá lo siguiente:

  • Posibilidad de seleccionar la enzima nativa o recombinante
  • Amplificación de productos de PCR con un tamaño máximo de 5 kb
  • Una enzima que posee licencia y está certificada para PCR

    Aplicaciones
    Amplificación de ADN de moldes genómicos, virales y plasmídicos complejos, transcriptasa inversa-reacción en cadena de la polimerasa (RT-PCR), ADNss de secuenciación y secuenciación de ciclos

    Fuente
    La enzima nativa se purifica de Thermus aquaticus YT1.

    Definición de la unidad
    Una unidad de ADN polimerasa Taq es la cantidad de enzima necesaria para incorporar 10 nmoles de desoxirribonucleótido en el ADN en 30 min a 74 °C.

    • Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

    Especificaciones
    Actividad exonucleasa5' - 3'
    Fidelidad (frente a Taq)1 X
    FormatoEnzima independiente
    Inicio en calienteNo
    N.º de reacciones500 reacciones
    Sobrante3'-A
    PolimerasaADN polimerasa Taq
    Tipo de productoADN polimerasa
    Cantidad500 unidades
    Formato de reacciónComponentes separados
    Condiciones de envíoAprobado para su envío en hielo húmedo o seco
    Tamaño (producto final)5 kb o menos
    Material de partidaADN
    Concentración5 U/μL
    Para utilizar con (aplicación)Standard PCR
    GC-Rich PCR PerformanceBajo
    Método de PCRqPCR, Standard PCR
    Velocidad de reacciónEstándar
    Unit SizeEach
    Contenido y almacenamiento
    Contiene:
    • 100 μl de ADN polimerasa Taq (5 U/μl)
    • Tampón para PCR 10X de 2,5 ml (sin magnesio)
    • 1 ml de cloruro magnésico (50 mm)

    Almacenar a -20 °C en un congelador que no forme escarcha. Estable durante seis meses si se almacena correctamente.

    Preguntas frecuentes

    My oligonucleotide does not appear to be the right length when I checked by gel electrophoresis. Why is this?

    Oligos should be run on a polyacrylamide gel containing 7 M urea and loaded with a 50% formamide solution to avoid compressions and secondary structures. Oligos of the same length and different compositions can electrophorese differently. dC's migrate fastest, followed by dA's, dT's, and then dG's. Oligos containing N's tend to run as a blurry band and generally have a problem with secondary structure.

    The primers I am using worked for PCR initially, but over time, have stopped working. What happened?

    Primers should be aliquoted for single use before PCR set-up. Heat just the aliquoted primers to 94 degrees for 1 min. Quick chill the primer on ice before adding to the PCR reaction. Some primers may anneal to themselves or curl up on themselves.

    I don't see a pellet in my oligo tube order. Should I ask for a replacement?

    The drying method dries the primer in a thin layer along the sidewalls of the tube instead of the bottom, therefore a pellet is not always visible and should still be ready to use.

    There is a ball-shaped pellet at the bottom of my oligo tube. What is this and can I still use my oligo?

    If the oligo was overheated, it will appear as a “ball”-shaped pellet attached to the bottom of the tube. This should not affect the quality of the oligo, and the oligo should be readily soluble in water.

    There is a green color in my lyophilized oligo. Can I still use it?

    If an oligo appears green in color, this is most likely due to ink falling into the tube. The oligo should still be fully functional. The color can be removed by doing an ethanol precipitation.

    View all Taq DNA Polymerase, native FAQs

    Citations & References (6)

    Citations & References
    Abstract
    Biosensor detection systems: engineering stable, high-affinity bioreceptors by yeast surface display.
    Authors:Richman SA, Kranz DM, Stone JD,
    Journal:Methods Mol Biol
    PubMed ID:19159105
    'Over the past two decades, the field of biosensors has been developing fast, portable, and convenient detection tools for various molecules of interest, both biological and environmental. Although much attention is paid to the transduction portion of the sensor, the actual bioreceptor that binds the ligand is equally critical. Tight, ... More
    Role of de novo DNA methyltransferases and methyl CpG-binding proteins in gene silencing in a rat hepatoma.
    Authors: Majumder Sarmila; Ghoshal Kalpana; Datta Jharna; Bai Shoumei; Dong Xiaocheng; Quan Ning; Plass Christoph; Jacob Samson T;
    Journal:J Biol Chem
    PubMed ID:11844796
    'The expression of metallothionein-I (MT-I), a known antioxidant, was suppressed in a transplanted rat hepatoma because of promoter methylation and was induced by heavy metals only after demethylation by 5-azacytidine (5-AzaC). Treatment of the tumor-bearing rats with 5-AzaC resulted in significant regression of the hepatoma. When the inhibitor-treated tumor was ... More
    Estrogen receptor alpha-mediated silencing of caveolin gene expression in neuronal cells.
    Authors:Zschocke J, Manthey D, Bayatti N, van der Burg B, Goodenough S, Behl C
    Journal:J Biol Chem
    PubMed ID:12138116
    'Estrogen receptors (ER alpha/ER beta) are expressed in neuronal cells and exhibit a variety of activities in the central nervous system. ER activity is regulated in a ligand-dependent manner and by co-regulatory factors. Caveolin-1 is a recently identified co-activator of ER alpha mediating the ligand-independent activation of this steroid receptor. ... More
    Allurin, a 21-kDa sperm chemoattractant from Xenopus egg jelly, is related to mammalian sperm-binding proteins.
    Authors: Olson J H; Xiang X; Ziegert T; Kittelson A; Rawls A; Bieber A L; Chandler D E;
    Journal:Proc Natl Acad Sci U S A
    PubMed ID:11562501
    Previously, we demonstrated that a protein from Xenopus egg jelly exhibits sperm chemoattractant activity when assayed by either video microscopy or by sperm passage across a porous filter. Here we describe the isolation and purification of allurin, the protein responsible for this activity. Freshly oviposited jellied eggs were soaked in ... More
    The polycystic kidney disease-1 promoter is a target of the beta-catenin/T-cell factor pathway.
    Authors:Rodova M, Islam MR, Maser RL, Calvet JP
    Journal:J Biol Chem
    PubMed ID:12048202
    Polycystic kidney disease (PKD) results from loss-of-function mutations in the PKD1 gene. There are also reports showing abnormally high levels of PKD1 expression in cystic epithelial cells. At present, nothing is known about the molecular mechanisms regulating the normal expression of the PKD1 gene or whether transcriptional disregulation of the ... More
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