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      Taq DNA Polymerase, native

      <i>Taq</i> DNA Polymerase, native

      Catalog number: 18038042

      Invitrogen™  Related applications: Chromatin Biology | Methylation Analysis | PCR

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      Description

      Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5´→3´ DNA polymerase activity and a 5´→3´ exonuclease activity.

      With our Taq DNA Polymerase, you get:

      • Your choice of recombinant or native enzyme
      • Amplification of PCR products up to 5 kb in size
      • An enzyme that is licensed and qualified for PCR

      Applications
      Amplification of DNA from complex genomic, viral, and plasmid templates, RT-PCR, sequencing ssDNA, and cycle sequencing

      Source
      Native enzyme is purified from Thermus aquaticus YT1.

      Unit definition
      One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of deoxyribonucleotide into DNA in 30 minutes at 74°C.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Polymerase: Taq DNA Polymerase
      Hot Start: No
      Fidelity (vs. Taq): 1 X
      GC-Rich PCR Performance: Low
      Reaction Format: Separate Components
      Product Overhang: 3'-A
      Reaction Speed: Standard
      Final Product Size(s): 5 kb or less
      Exonuclease Activity: 5' - 3'
      Product Size: 500 units
      Shipping Condition: Approved for shipment on Wet or Dry Ice

      Contents & storage

      Contains:
      • 100 µl Taq DNA Polymerase (5 U/µl)
      • 2.5 ml 10X PCR buffer (without magnesium)
      • 1 ml Magnesium Chloride (50 mM)

      Store at -20°C in a non-frost-free freezer. Stable for 6 months when stored properly.

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