This product is manufactured in our Grand Island, US facility and is intended for sale in North America, Latin America, and the Asia/Pacific region—higher freight charges will apply to orders shipped elsewhere. An equivalent product, manufactured in Paisley, Scotland, is recommended for our customers in Europe, Africa, and the Middle East. Click to view.

CO2 Independent Medium This product, and/or its packaging, incorporates one or more environmental/green features. Click on this icon to learn more.

Catalog number:  18045088

Related applications:

Mammalian Cell Culture

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CO2 Independent Medium is a non-HEPES proprietary medium used for supporting cell growth for a variety of suspension and adherent mammalian cells such as epithelial, fibroblast, and lymphoid cell lines without a CO2 incubator. CO2 Independent Medium is ideal for transporting cells or tissue and for the handling of mouse embryos under atmospheric conditions.
This CO2 Independent Medium is manufactured as follows:
• Phenol Red• L-glutamine
• Sodium pyruvate• HEPES

The complete formulation is confidential. For more information, please contact Technical Support.

Gibco® CO2 Independent Medium contains a unique buffering system composed of mono and dibasic sodium phosphate and β-glycerophosphate. A small amount of sodium bicarbonate has been included in the formulation to meet essential bicarbonate dependent functions. No synthetic buffers are utilized, thus eliminating any cytotoxic effects associated with such buffering systems. Additionally, Gibco® CO2 Independent Medium has been formulated with components that enhance cellular production and utilization of CO2 such that an exogenous source of CO2 is not required for the maintenance of CO2 dependent cellular functions.

Product Use
For research use only: Not intended for human or animal diagnostic or therapeutic uses.

Dual-Site cGMP Manufacturing
Gibco® CO2 Independent Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical Gibco® CO2 Independent Medium product made in our Scotland facility (18045-054). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

CO2 Independent Medium contains no lipids or growth factors. Therefore, CO2 Independent Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). The CO2 Independent Medium does not require a CO2 environment to maintain physiological pH. For maximum growth performance some cell lines may require either direct or sequential adaptation to CO2 Independent Medium (see protocol).
For Research Use Only. Not for use in diagnostic procedures.


Shipping Condition: Room Temperature
Volume (Metric): 500 ml
Form: Liquid
Glutamine: No Glutamine
Green Features: Sustainable packaging
Quantity: 500mL

Contents & storage

Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture