CO₂ Independent Medium is a non-HEPES proprietary medium used for supporting cell growth for a variety of suspension and adherent mammalian cells such as epithelial, fibroblast, and lymphoid cell lines without a CO₂ incubator. CO₂ Independent Medium is ideal for transporting cells or tissue and for the handling of mouse embryos under atmospheric conditions.
The complete formulation is confidential. For more information, please contact Technical Support.

Gibco™ CO₂ Independent Medium contains a unique buffering system composed of mono and dibasic sodium phosphate and β-glycerophosphate. A small amount of sodium bicarbonate has been included in the formulation to meet essential bicarbonate dependent functions. No synthetic buffers are utilized, thus eliminating any cytotoxic effects associated with such buffering systems. Additionally, Gibco™ CO₂ Independent Medium has been formulated with components that enhance cellular production and utilization of CO₂ such that an exogenous source of CO₂ is not required for the maintenance of CO₂ dependent cellular functions.

Product Use
For research use only: Not intended for human or animal diagnostic or therapeutic uses.

Dual-Site cGMP Manufacturing
Gibco™ CO₂ Independent Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, Life Technologies offers an identical Gibco™ CO₂ Independent Medium product made in our Scotland facility (18045-054). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

CO₂ Independent Medium contains no lipids or growth factors. Therefore, CO₂ Independent Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). The CO₂ Independent Medium does not require a CO₂ environment to maintain physiological pH. For maximum growth performance some cell lines may require either direct or sequential adaptation to CO₂ Independent Medium (see protocol).