Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA.
SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.
Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.
For Research Use Only. Not for use in diagnostic procedures.
RNA dependent DNA Polymerase
SuperScript II Reverse Transcriptase (10,000 units total, at 200U/μL), 5X first-strand buffer [250mM Tris-HCl (pH 8.3), 375mM KCl, 15mM MgCl2], and 100mM DTT
Optimal Reaction Temperature
50 reactions (10,000 units)
Ribonuclease H Activity
GC-Rich PCR Performance
No. of Reactions
No. of Tests
Product Type Specs
Size (Final Product)
12.3 kb or less
Contents & Storage
SuperScript™ II Reverse Transcriptase (10,000 units total, at 200U/µL) is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.