For superior cDNA synthesis performance upgrade to Invitrogen™ SuperScript™ IV Reverse Transcriptase.
Invitrogen™

SuperScript™ II Reverse Transcriptase

Catalog number:  18064071

Related applications:

Reverse Transcription

Back to top

Description

Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT. Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA.

SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date.

Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase, features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Concentration: 200 U⁄µl
Enzyme Function: RNA dependent DNA Polymerase
Final Product Size(s): 12.3 kb or less
Form: Frozen
Format: Tube(s)
Number of Reactions: 200 reactions
Optimal Reaction Temperature: 42° C
Product Line: SuperScript™
Product Size: 4 x 10,000 units
Reverse Transcriptase: SuperScript™ II
Ribonuclease H Activity: Reduced

Contents & storage

SuperScript™ II Reverse Transcriptase (4 × 10,000 units total, at 200U/µL) is supplied with a vial of 5X first-strand buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2], and a vial of 100 mM DTT.