Invitrogen™

DNase I, Amplification Grade

Catalog number:  18068015

Related applications:

Reverse Transcription

| Real Time PCR (qPCR)

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Description

DNase I, Amplification Grade, digests single- and double-stranded DNA to oligodexyribonuleotides containing a 5' phosphate. DNase I, Amplification Grade, is suitable for eliminating DNA during critical RNA purification procedures such as those prior to RNA-PCR amplification. It is purified and tested for non-detectable levels of RNase contamination. Absence of RNase is tested by performing a ribonuclease assay with RNA ladder.

Application
Removing DNA from RNA and protein preparations.

Specific activity
Specific activity is >10,000 units/mg.

Source
Purified from bovine pancreas

Performance and quality testing
Ribonuclease assay with RNA ladder and ability to digest single-stranded and double-stranded DNA to oligonucleotides are determined.

Unit definition
One unit increases the absorbance of a high molecular weight DNA solution at a rate of 0.001 A260 units/min/mL of reaction mixture at 25°C.

Unit reaction conditions
0.1 M sodium acetate (pH 5.0), 5 mM MgCl2, 50 µg/mL calf thymus DNA, and enzyme in 1 mL for 10 min at 25°C.
WARNING: Reproductive Harm - www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Shipping Condition: Approved for shipment on Wet or Dry Ice
Enzyme: DNase

Contents & storage

Contents:
1 vial of DNase I, Amp Grade (100 U)
1 vial of 10X DNase I Reaction Buffer (1000 µL)
1 vial of 25 mM EDTA (pH 8.0) (200 µL)

Store at -20°C in a non-frost-free freezer.